eprintid: 10161211 rev_number: 11 eprint_status: archive userid: 699 dir: disk0/10/16/12/11 datestamp: 2022-12-02 11:36:30 lastmod: 2024-11-07 15:15:35 status_changed: 2022-12-02 11:36:30 type: article metadata_visibility: show sword_depositor: 699 creators_name: Hoshino, Yukiko creators_name: Takechi, Masaki creators_name: Moazen, Mehran creators_name: Steacy, Miranda creators_name: Koyabu, Daisuke creators_name: Furutera, Toshiko creators_name: Ninomiya, Youichirou creators_name: Nuri, Takashi creators_name: Pauws, Erwin creators_name: Iseki, Sachiko title: Synchondrosis fusion contributes to the progression of postnatal craniofacial dysmorphology in syndromic craniosynostosis ispublished: pub subjects: GOSH divisions: UCL divisions: B02 divisions: B04 divisions: C05 divisions: D13 divisions: F45 divisions: G22 keywords: Apert syndrome, coronal suture, craniosynostosis, Crouzon syndrome, geometric morphometrics, inter-sphenoid synchondrosis, midfacial hypoplasia, Saethre-Chotzen syndrome note: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions. abstract: Syndromic craniosynostosis (CS) patients exhibit early, bony fusion of calvarial sutures and cranial synchondroses, resulting in craniofacial dysmorphology. In this study, we chronologically evaluated skull morphology change after abnormal fusion of the sutures and synchondroses in mouse models of syndromic CS for further understanding of the disease. We found fusion of the inter-sphenoid synchondrosis (ISS) in Apert syndrome model mice (Fgfr2S252W/+ ) around 3 weeks old as seen in Crouzon syndrome model mice (Fgfr2cC342Y/+ ). We then examined ontogenic trajectories of CS mouse models after 3 weeks of age using geometric morphometrics analyses. Antero-ventral growth of the face was affected in Fgfr2S252W/+ and Fgfr2cC342Y/+ mice, while Saethre-Chotzen syndrome model mice (Twist1+/- ) did not show the ISS fusion and exhibited a similar growth pattern to that of control littermates. Further analysis revealed that the coronal suture synostosis in the CS mouse models induces only the brachycephalic phenotype as a shared morphological feature. Although previous studies suggest that the fusion of the facial sutures during neonatal period is associated with midface hypoplasia, the present study suggests that the progressive postnatal fusion of the cranial synchondrosis also contributes to craniofacial dysmorphology in mouse models of syndromic CS. These morphological trajectories increase our understanding of the progression of syndromic CS skull growth. date: 2023-03 date_type: published publisher: Wiley-Blackwell official_url: https://doi.org/10.1111/joa.13790 oa_status: green full_text_type: other language: eng primo: open primo_central: open_green verified: verified_manual elements_id: 1991727 doi: 10.1111/joa.13790 lyricists_name: Moazen, Mehran lyricists_name: Pauws, Erwin lyricists_id: MMOAZ42 lyricists_id: EPAUW82 actors_name: Pauws, Erwin actors_id: EPAUW82 actors_role: owner funding_acknowledgements: EP/W008092/1 [Engineering and Physical Sciences Research Council] full_text_status: public publication: Journal of Anatomy volume: 242 number: 3 event_location: England issn: 0021-8782 citation: Hoshino, Yukiko; Takechi, Masaki; Moazen, Mehran; Steacy, Miranda; Koyabu, Daisuke; Furutera, Toshiko; Ninomiya, Youichirou; ... Iseki, Sachiko; + view all <#> Hoshino, Yukiko; Takechi, Masaki; Moazen, Mehran; Steacy, Miranda; Koyabu, Daisuke; Furutera, Toshiko; Ninomiya, Youichirou; Nuri, Takashi; Pauws, Erwin; Iseki, Sachiko; - view fewer <#> (2023) Synchondrosis fusion contributes to the progression of postnatal craniofacial dysmorphology in syndromic craniosynostosis. Journal of Anatomy , 242 (3) 10.1111/joa.13790 <https://doi.org/10.1111/joa.13790>. Green open access document_url: https://discovery.ucl.ac.uk/id/eprint/10161211/1/Hoshino%20et%20al_JAnat_Accepted%20version.pdf