eprintid: 10157479 rev_number: 9 eprint_status: archive userid: 699 dir: disk0/10/15/74/79 datestamp: 2022-10-19 15:39:35 lastmod: 2022-10-19 15:39:35 status_changed: 2022-10-19 15:39:35 type: article metadata_visibility: show sword_depositor: 699 creators_name: Michno, Wojciech creators_name: Koutarapu, Srinivas creators_name: Camacho, Rafael creators_name: Toomey, Christina creators_name: Stringer, Katie creators_name: Minta, Karolina creators_name: Ge, Junyue creators_name: Jha, Durga creators_name: Fernandez-Rodriguez, Julia creators_name: Brinkmalm, Gunnar creators_name: Zetterberg, Henrik creators_name: Blennow, Kaj creators_name: Ryan, Natalie S creators_name: Lashley, Tammaryn creators_name: Hanrieder, Jörg title: Chemical traits of cerebral amyloid angiopathy in familial British-, Danish-, and non-Alzheimerʼs dementias ispublished: inpress divisions: C07 divisions: F84 divisions: B02 divisions: UCL divisions: D07 divisions: F86 keywords: ABri, ADan, Alzheimer's disease (AD), cerebral amyloid angiopathy (CAA), familial British dementia (FBD), familial Danish dementia (FDD) note: © 2022 The Authors. Journal of Neurochemistry published by John Wiley & Sons Ltd on behalf of International Society for Neurochemistry. This is an open access article under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/). abstract: Familial British dementia (FBD) and familial Danish dementia (FDD) are autosomal dominant forms of dementia caused by mutations in the integral membrane protein 2B (ITM2B, also known as BRI2) gene. Secretase processing of mutant BRI2 leads to secretion and deposition of BRI2-derived amyloidogenic peptides, ABri and ADan that resemble APP/β-amyloid (Aβ) pathology, which is characteristic of Alzheimer's disease (AD). Amyloid pathology in FBD/FDD manifests itself predominantly in the microvasculature by ABri/ADan containing cerebral amyloid angiopathy (CAA). While ABri and ADan peptide sequences differ only in a few C-terminal amino acids, CAA in FDD is characterized by co-aggregation of ADan with Aβ, while in contrast no Aβ deposition is observed in FBD. The fact that FDD patients display an earlier and more severe disease onset than FBD suggests a potential role of ADan and Aβ co-aggregation that promotes a more rapid disease progression in FDD compared to FBD. It is therefore critical to delineate the chemical signatures of amyloid aggregation in these two vascular dementias. This in turn will increase the knowledge on the pathophysiology of these diseases and the pathogenic role of heterogenous amyloid peptide interactions and deposition, respectively. Herein, we used matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) in combination with hyperspectral, confocal microscopy based on luminescent conjugated oligothiophene probes (LCO) to delineate the structural traits and associated amyloid peptide patterns of single CAA in postmortem brain tissue of patients with FBD, FDD as well as sporadic CAA without AD (CAA+) that show pronounced CAA without parenchymal plaques. The results show that CAA in both FBD and FDD consist of N-terminally truncated- and pyroglutamate-modified amyloid peptide species (ADan and ABri), but that ADan peptides in FDD are also extensively C-terminally truncated as compared to ABri in FBD, which contributes to hydrophobicity of ADan species. Further, CAA in FDD showed co-deposition with Aβ x-42 and Aβ x-40 species. CAA+ vessels were structurally more mature than FDD/FBD CAA and contained significant amounts of pyroglutamated Aβ. When compared with FDD, Aβ in CAA+ showed more C-terminal and less N-terminally truncations. In FDD, ADan showed spatial co-localization with Aβ3pE-40 and Aβ3-40 but not with Aβx-42 species. This suggests an increased aggregation propensity of Aβ in FDD that promotes co-aggregation of both Aβ and ADan. Further, CAA maturity appears to be mainly governed by Aβ content based on the significantly higher 500/580 patterns observed in CAA+ than in FDD and FBD, respectively. Together this is the first study of its kind on comprehensive delineation of Bri2 and APP-derived amyloid peptides in single vascular plaques in both FDD/FBD and sporadic CAA that provides new insight in non-AD-related vascular amyloid pathology. (Figure presented.) date: 2022-09-25 date_type: published publisher: Wiley official_url: https://doi.org/10.1111/jnc.15694 oa_status: green full_text_type: pub language: eng primo: open primo_central: open_green verified: verified_manual elements_id: 1977316 doi: 10.1111/jnc.15694 medium: Print-Electronic lyricists_name: Toomey, Christina lyricists_name: Zetterberg, Henrik lyricists_name: Hanrieder, Jorg lyricists_name: Ryan, Natalie lyricists_name: Lashley, Tammaryn lyricists_id: CEMUR42 lyricists_id: HZETT94 lyricists_id: JHANR44 lyricists_id: NRYAN07 lyricists_id: TJOHN05 actors_name: Zetterberg, Henrik actors_name: Harriot, Anne-Marie actors_id: HZETT94 actors_id: AHARA72 actors_role: owner actors_role: impersonator funding_acknowledgements: 2018-02181 [Vetenskapsradet]; 2019-02397 [Vetenskapsradet] full_text_status: public publication: Journal of Neurochemistry pages: 14 event_location: England citation: Michno, Wojciech; Koutarapu, Srinivas; Camacho, Rafael; Toomey, Christina; Stringer, Katie; Minta, Karolina; Ge, Junyue; ... Hanrieder, Jörg; + view all <#> Michno, Wojciech; Koutarapu, Srinivas; Camacho, Rafael; Toomey, Christina; Stringer, Katie; Minta, Karolina; Ge, Junyue; Jha, Durga; Fernandez-Rodriguez, Julia; Brinkmalm, Gunnar; Zetterberg, Henrik; Blennow, Kaj; Ryan, Natalie S; Lashley, Tammaryn; Hanrieder, Jörg; - view fewer <#> (2022) Chemical traits of cerebral amyloid angiopathy in familial British-, Danish-, and non-Alzheimerʼs dementias. Journal of Neurochemistry 10.1111/jnc.15694 <https://doi.org/10.1111/jnc.15694>. (In press). Green open access document_url: https://discovery.ucl.ac.uk/id/eprint/10157479/2/Zetterberg_Chemical%20traits%20of%20cerebral%20amyloid%20angiopathy%20in%20familial%20British-%2C%20Danish-%2C%20and%20non-Alzheimer%CA%BCs%20dementias_AOP.pdf