eprintid: 10156242
rev_number: 8
eprint_status: archive
userid: 699
dir: disk0/10/15/62/42
datestamp: 2022-09-27 10:57:27
lastmod: 2022-09-27 10:57:27
status_changed: 2022-09-27 10:57:27
type: article
metadata_visibility: show
sword_depositor: 699
creators_name: Tromer, Eelco C
creators_name: Wemyss, Thomas A
creators_name: Ludzia, Patryk
creators_name: Waller, Ross F
creators_name: Akiyoshi, Bungo
title: Repurposing of synaptonemal complex proteins for kinetochores in Kinetoplastida
ispublished: pub
divisions: C05
divisions: F48
divisions: B04
divisions: UCL
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abstract: Chromosome segregation in eukaryotes is driven by the kinetochore, a macromolecular complex that connects centromeric DNA to microtubules of the spindle apparatus. Kinetochores in well-studied model eukaryotes consist of a core set of proteins that are broadly conserved among distant eukaryotic phyla. By contrast, unicellular flagellates of the class Kinetoplastida have a unique set of 36 kinetochore components. The evolutionary origin and history of these kinetochores remain unknown. Here, we report evidence of homology between axial element components of the synaptonemal complex and three kinetoplastid kinetochore proteins KKT16-18. The synaptonemal complex is a zipper-like structure that assembles between homologous chromosomes during meiosis to promote recombination. By using sensitive homology detection protocols, we identify divergent orthologues of KKT16-18 in most eukaryotic supergroups, including experimentally established chromosomal axis components, such as Red1 and Rec10 in budding and fission yeast, ASY3-4 in plants and SYCP2-3 in vertebrates. Furthermore, we found 12 recurrent duplications within this ancient eukaryotic SYCP
            2–3
            gene family, providing opportunities for new functional complexes to arise, including KKT16-18 in the kinetoplastid parasite
            Trypanosoma brucei
            . We propose the kinetoplastid kinetochore system evolved by repurposing meiotic components of the chromosome synapsis and homologous recombination machinery that were already present in early eukaryotes.
date: 2021-05
date_type: published
publisher: The Royal Society
official_url: https://doi.org/10.1098/rsob.210049
oa_status: green
full_text_type: pub
language: eng
primo: open
primo_central: open_green
verified: verified_manual
elements_id: 1978760
doi: 10.1098/rsob.210049
lyricists_name: Wemyss, Thomas
lyricists_id: TAWEM65
actors_name: Wemyss, Thomas
actors_id: TAWEM65
actors_role: owner
full_text_status: public
publication: Open Biology
volume: 11
number: 5
article_number: 210049
citation:        Tromer, Eelco C;    Wemyss, Thomas A;    Ludzia, Patryk;    Waller, Ross F;    Akiyoshi, Bungo;      (2021)    Repurposing of synaptonemal complex proteins for kinetochores in Kinetoplastida.                   Open Biology , 11  (5)    , Article 210049.  10.1098/rsob.210049 <https://doi.org/10.1098/rsob.210049>.       Green open access   
 
document_url: https://discovery.ucl.ac.uk/id/eprint/10156242/1/rsob.210049.pdf