eprintid: 10156004
rev_number: 8
eprint_status: archive
userid: 699
dir: disk0/10/15/60/04
datestamp: 2022-09-22 09:20:20
lastmod: 2023-09-16 06:10:07
status_changed: 2022-09-22 09:20:20
type: article
metadata_visibility: show
sword_depositor: 699
creators_name: Barkus, Chris
creators_name: Bergmann, Caroline
creators_name: Branco, Tiago
creators_name: Carandini, Matteo
creators_name: Chadderton, Paul T
creators_name: Galiñanes, Gregorio L
creators_name: Gilmour, Gary
creators_name: Huber, Daniel
creators_name: Huxter, John R
creators_name: Khan, Adil G
creators_name: King, Andrew J
creators_name: Maravall, Miguel
creators_name: O'Mahony, Tina
creators_name: Ragan, C Ian
creators_name: Robinson, Emma SJ
creators_name: Schaefer, Andreas T
creators_name: Schultz, Simon R
creators_name: Sengpiel, Frank
creators_name: Prescott, Mark J
title: Refinements to rodent head fixation and fluid/food control for neuroscience
ispublished: pub
divisions: C07
divisions: D08
divisions: B02
divisions: UCL
divisions: C08
divisions: G02
divisions: D09
keywords: 3Rs, Animal welfare, Head-fixation, Mice, Restraint, Water restriction
note: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
abstract: The use of head fixation in mice is increasingly common in research, its use having initially been restricted to the field of sensory neuroscience. Head restraint has often been combined with fluid control, rather than food restriction, to motivate behaviour, but this too is now in use for both restrained and non-restrained animals. Despite this, there is little guidance on how best to employ these techniques to optimise both scientific outcomes and animal welfare. This article summarises current practices and provides recommendations to improve animal wellbeing and data quality, based on a survey of the community, literature reviews, and the expert opinion and practical experience of an international working group convened by the UK's National Centre for the Replacement, Refinement and Reduction of Animals in Research (NC3Rs). Topics covered include head fixation surgery and post-operative care, habituation to restraint, and the use of fluid/food control to motivate performance. We also discuss some recent developments that may offer alternative ways to collect data from large numbers of behavioural trials without the need for restraint. The aim is to provide support for researchers at all levels, animal care staff, and ethics committees to refine procedures and practices in line with the refinement principle of the 3Rs.
date: 2022-09-09
date_type: published
publisher: Elsevier BV
official_url: https://doi.org/10.1016/j.jneumeth.2022.109705
oa_status: green
full_text_type: other
language: eng
primo: open
primo_central: open_green
verified: verified_manual
elements_id: 1977780
doi: 10.1016/j.jneumeth.2022.109705
medium: Print-Electronic
pii: S0165-0270(22)00231-X
lyricists_name: Carandini, Matteo
lyricists_name: Schaefer, Andreas
lyricists_id: MCARA89
lyricists_id: ASCHA85
actors_name: Carandini, Matteo
actors_id: MCARA89
actors_role: owner
full_text_status: public
publication: Journal of Neuroscience Methods
volume: 381
article_number: 109705
event_location: Netherlands
citation:        Barkus, Chris;    Bergmann, Caroline;    Branco, Tiago;    Carandini, Matteo;    Chadderton, Paul T;    Galiñanes, Gregorio L;    Gilmour, Gary;                                                 ... Prescott, Mark J; + view all <#>        Barkus, Chris;  Bergmann, Caroline;  Branco, Tiago;  Carandini, Matteo;  Chadderton, Paul T;  Galiñanes, Gregorio L;  Gilmour, Gary;  Huber, Daniel;  Huxter, John R;  Khan, Adil G;  King, Andrew J;  Maravall, Miguel;  O'Mahony, Tina;  Ragan, C Ian;  Robinson, Emma SJ;  Schaefer, Andreas T;  Schultz, Simon R;  Sengpiel, Frank;  Prescott, Mark J;   - view fewer <#>    (2022)    Refinements to rodent head fixation and fluid/food control for neuroscience.                   Journal of Neuroscience Methods , 381     , Article 109705.  10.1016/j.jneumeth.2022.109705 <https://doi.org/10.1016/j.jneumeth.2022.109705>.       Green open access   
 
document_url: https://discovery.ucl.ac.uk/id/eprint/10156004/1/PROTECT%20-%20RESEARCH%20Refinements%20to%20mouse%20high%20yield%20behavioural%20experiments.pdf