TY  - INPR
KW  - CD142
KW  -  adipogenesis regulators (Aregs)
KW  -  adipose stem and precursor cells
KW  -  differentiation
KW  -  retinoic acid
A1  - Zachara, Magda
A1  - Rainer, Pernille Y
A1  - Hashimi, Horia
A1  - Russeil, Julie M
A1  - Alpern, Daniel
A1  - Ferrero, Radiana
A1  - Litovchenko, Maria
A1  - Deplancke, Bart
JF  - The EMBO Journal
SN  - 0261-4189
PB  - EMBO
UR  - https://doi.org/10.15252/embj.2021108206
ID  - discovery10154771
N2  - Adipose stem and precursor cells (ASPCs) give rise to adipocytes and determine the composition and plasticity of adipose tissue. Recently, several studies have demonstrated that ASPCs partition into at least three distinct cell subpopulations, including the enigmatic CD142+ cells. An outstanding challenge is to functionally characterise this population, as discrepant properties, from adipogenic to non- and anti-adipogenic, have been reported for these cells. To resolve these phenotypic ambiguities, we characterised mammalian subcutaneous CD142+ ASPCs across various experimental conditions, demonstrating that CD142+ ASPCs exhibit high molecular and phenotypic robustness. Specifically, we find these cells to be firmly non- and anti-adipogenic both in?vitro and in?vivo, with their inhibitory signals also impacting adipogenic human cells. However, these CD142+ ASPC-specific properties exhibit surprising temporal phenotypic alterations, and emerge only in an age-dependent manner. Finally, using multi-omic and functional assays, we show that the inhibitory nature of these adipogenesis-regulatory CD142+ ASPCs (Aregs) is driven by specifically expressed secretory factors that cooperate with the retinoic acid signalling pathway to transform the adipogenic state of CD142- ASPCs into a non-adipogenic, Areg-like state.
N1  - Copyright © 2022 The Authors. Published under the terms of the CC BY NC ND 4.0 license. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
AV  - public
Y1  - 2022/08/22/
TI  - Mammalian adipogenesis regulator (Areg) cells use retinoic acid signalling to be non- and anti-adipogenic in age-dependent manner
ER  -