eprintid: 10151845 rev_number: 22 eprint_status: archive userid: 699 dir: disk0/10/15/18/45 datestamp: 2022-07-12 09:32:54 lastmod: 2024-10-25 15:45:38 status_changed: 2022-12-08 17:33:28 type: article metadata_visibility: show sword_depositor: 699 creators_name: Ruijs, Titia Q creators_name: Koopmans, Ingrid W creators_name: de Kam, Marieke L creators_name: van Esdonk, Michiel J creators_name: Koltzenburg, Martin creators_name: Groeneveld, Geert Jan creators_name: Heuberger, Jules AAC title: Effects of mexiletine and lacosamide on nerve excitability in healthy subjects: a randomized, double-blind, placebo-controlled, crossover study ispublished: pub subjects: UCH divisions: C07 divisions: F84 divisions: B02 divisions: UCL divisions: D07 note: This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. See: http://creativecommons.org/licenses/by-nc-nd/4.0/ abstract: Selective voltage-gated sodium channel blockers are of growing interest as treatment for pain. For drug development of such compounds, it would be critical to have a biomarker that can be used for proof-of-mechanism. We aimed to evaluate whether drug-induced changes in sodium conductance can be detected in the peripheral nerve excitability profile in 18 healthy subjects. In a randomized, double-blind, 3-way crossover study, effects of single oral doses of 333 mg mexiletine and 300 mg lacosamide were compared with placebo. On each study visit, motor and sensory nerve excitability measurements of the median nerve were performed (predose; and 3 and 6 hours postdose) using Qtrac. Treatment effects were calculated using an analysis of covariance (ANCOVA) with baseline as covariate. Mexiletine and lacosamide had significant effects on multiple motor and sensory nerve excitability variables. Depolarizing threshold electrotonus (TEd40 (40–60 ms)) decreased by mexiletine (estimated difference (ED) −1.37% (95% confidence interval (CI): −2.20, −0.547; P = 0.002) and lacosamide (ED −1.27%, 95% CI: −2.10, −0.443; P = 0.004) in motor nerves. Moreover, mexiletine and lacosamide decreased superexcitability (less negative) in motor nerves (ED 1.74%, 95% CI: 0.615, 2.87; P = 0.004, and ED 1.47%, 95% CI: 0.341, 2.60; P = 0.013, respectively). Strength-duration time constant decreased after lacosamide in motor- (ED −0.0342 ms, 95% CI: −0.0571, −0.0112; P = 0.005) and sensory nerves (ED −0.0778 ms, 95% CI: −0.116, −0.0399; P < 0.001). Mexiletine and lacosamide significantly decrease excitability of motor and sensory nerves, in line with their suggested mechanism of action. Results of this study indicate that nerve excitability threshold tracking can be an effective pharmacodynamic biomarker. The method could be a valuable tool in clinical drug development. date: 2022-07-19 date_type: published publisher: Wiley official_url: https://doi.org/10.1002/cpt.2694 oa_status: green full_text_type: pub language: eng primo: open primo_central: open_green verified: verified_manual elements_id: 1964458 doi: 10.1002/cpt.2694 medium: Print-Electronic lyricists_name: Koltzenburg, Martin lyricists_id: MKOLT33 actors_name: Koltzenburg, Martin actors_id: MKOLT33 actors_role: owner full_text_status: public publication: Clinical Pharmacology & Therapeutics volume: 112 number: 5 pagerange: 1008-1019 event_location: United States citation: Ruijs, Titia Q; Koopmans, Ingrid W; de Kam, Marieke L; van Esdonk, Michiel J; Koltzenburg, Martin; Groeneveld, Geert Jan; Heuberger, Jules AAC; (2022) Effects of mexiletine and lacosamide on nerve excitability in healthy subjects: a randomized, double-blind, placebo-controlled, crossover study. Clinical Pharmacology & Therapeutics , 112 (5) pp. 1008-1019. 10.1002/cpt.2694 <https://doi.org/10.1002/cpt.2694>. Green open access document_url: https://discovery.ucl.ac.uk/id/eprint/10151845/1/Koltzenburg_Clin%20Pharma%20and%20Therapeutics%20-%202022%20-%20Ruijs%20-%20Effects%20of%20Mexiletine%20and%20Lacosamide%20on%20Nerve%20Excitability%20in%20Healthy.pdf