eprintid: 10147804 rev_number: 9 eprint_status: archive userid: 699 dir: disk0/10/14/78/04 datestamp: 2022-05-04 17:07:11 lastmod: 2022-05-04 17:07:20 status_changed: 2022-05-04 17:07:11 type: article metadata_visibility: show sword_depositor: 699 creators_name: Hailes, Helen creators_name: Carter, Eve creators_name: Ambrose-Dempster, Esther creators_name: Ward, John M creators_name: Sheppard, Tom title: Mechanoenzymatic reactions with whole cell transaminases: shaken, not stirred ispublished: inpress divisions: C06 divisions: F56 divisions: B04 divisions: UCL note: This article is licensed under a Creative Commons Attribution 3.0 Unported Licence (http://creativecommons.org/licenses/by/3.0/). abstract: Mechanochemical reactions have emerged in recent years as a green synthetic method because reactions can be performed more rapidly and using less solvent than traditional synthetic approaches. To date, very few mechanoenzymatic reactions have been described. For the first time, transaminases, which are widely used for the amination of aldehydes and ketones, have been used here under mechanoenzymatic conditions to produce amines using significantly less aqueous medium than conventional biocatalytic reactions. The direct use of whole cells was also possible and shorter reaction times could be used to provide amines efficiently with high yields and stereoselectivities. date: 2022-04-11 date_type: published publisher: Royal Society of Chemistry (RSC) official_url: https://doi.org/10.1039/d2gc01006b oa_status: green full_text_type: pub language: eng primo: open primo_central: open_green verified: verified_manual elements_id: 1952435 doi: 10.1039/d2gc01006b lyricists_name: Sheppard, Thomas lyricists_id: TSHEP42 actors_name: Barczynska, Patrycja actors_id: PBARC91 actors_role: owner full_text_status: public publication: Green Chemistry citation: Hailes, Helen; Carter, Eve; Ambrose-Dempster, Esther; Ward, John M; Sheppard, Tom; (2022) Mechanoenzymatic reactions with whole cell transaminases: shaken, not stirred. Green Chemistry 10.1039/d2gc01006b <https://doi.org/10.1039/d2gc01006b>. (In press). Green open access document_url: https://discovery.ucl.ac.uk/id/eprint/10147804/1/Sheppard_d2gc01006b.pdf