TY - JOUR SP - 2407 N2 - BACKGROUND AND AIMS: The accumulation of fat increases the formation of lipid peroxides, which are partly scavenged by alpha-tocopherol (?-TOH). Here, we aimed to investigate the associations between different measures of (abdominal) fat and levels of urinary ?-TOH metabolites in middle-aged individuals. METHODS AND RESULTS: In this cross-sectional analysis in the Netherlands Epidemiology of Obesity study (N = 511, 53% women; mean [SD] age of 55 [6.1] years), serum ?-TOH and ?-TOH metabolites from 24-h urine were measured as alpha-tocopheronolactone hydroquinone (?-TLHQ, oxidized) and alpha-carboxymethyl-hydroxychroman (?-CEHC, enzymatically converted) using liquid-chromatography-tandem mass spectrometry. Body mass index and total body fat were measured, and abdominal subcutaneous and visceral adipose tissue (aSAT and VAT) were assessed using magnetic resonance imaging. Using multivariable-adjusted linear regression analyses, we analysed the associations of BMI, TBF, aSAT and VAT with levels of urinary ?-TOH metabolites, adjusted for confounders. We observed no evidence for associations between body fat measures and serum ?-TOH. Higher BMI and TBF were associated with lower urinary levels of TLHQ (0.95 [95%CI: 0.90, 1.00] and 0.94 [0.88, 1.01] times per SD, respectively) and with lower TLHQ relative to CEHC (0.93 [0.90, 0.98] and 0.93 [0.87, 0.98] times per SD, respectively). We observed similar associations for VAT (TLHQ: 0.94 [0.89, 0.99] times per SD), but not for aSAT. CONCLUSIONS: Opposite to our research hypothesis, higher abdominal adiposity was moderately associated with lower levels of oxidized ?-TOH metabolites, which might reflect lower vitamin E antioxidative activity in individuals with higher abdominal fat instead. A1 - Meulmeester, Fleur L A1 - Luo, Jiao A1 - Martens, Leon G A1 - Ashrafi, Nadia A1 - de Mutsert, Renee A1 - Mook-Kanamori, Dennis O A1 - Lamb, Hildo J A1 - Rosendaal, Frits R A1 - van Dijk, Ko Willems A1 - Mills, Kevin A1 - van Heemst, Diana A1 - Noordam, Raymond Y1 - 2021/07/09/ UR - https://doi.org/10.1016/j.numecd.2021.05.001 TI - Association of measures of body fat with serum alpha-tocopherol and its metabolites in middle-aged individuals ID - discovery10146106 AV - public KW - Science & Technology KW - Life Sciences & Biomedicine KW - Cardiac & Cardiovascular Systems KW - Endocrinology & Metabolism KW - Nutrition & Dietetics KW - Cardiovascular System & Cardiology KW - Obesity KW - Visceral adipose tissue KW - Subcutaneous adipose tissue KW - Vitamin E metabolites KW - Alpha tocopherol KW - Mass spectrometry KW - Epidemiology KW - OXIDATIVE STRESS KW - VITAMIN-E KW - OBESITY KW - EXPRESSION KW - PALMITATE KW - ADULTS KW - ACIDS EP - 2415 N1 - © 2021 The Author(s). Published by Elsevier B.V. on behalf of The Italian Diabetes Society, the Italian Society for the Study of Atherosclerosis, the Italian Society of Human Nutrition and the Department of Clinical Medicine and Surgery, Federico II University. This is an open access article under the CC BY license (http://creativecommons. org/licenses/by/4.0/). JF - Nutrition, Metabolism & Cardiovascular Diseases IS - 8 VL - 31 PB - ELSEVIER SCI LTD ER -