eprintid: 10135179
rev_number: 13
eprint_status: archive
userid: 608
dir: disk0/10/13/51/79
datestamp: 2021-09-28 16:13:46
lastmod: 2021-09-28 16:13:46
status_changed: 2021-09-28 16:13:46
type: article
metadata_visibility: show
creators_name: Richardson, KH
creators_name: Wright, JJ
creators_name: Simenas, M
creators_name: Thiemann, J
creators_name: Esteves, AM
creators_name: McGuire, G
creators_name: Myers, WK
creators_name: Morton, JJL
creators_name: Hippler, M
creators_name: Nowaczyk, MM
creators_name: Hanke, GT
creators_name: Roessler, MM
title: Functional basis of electron transport within photosynthetic complex I
ispublished: pub
divisions: UCL
divisions: B04
divisions: C06
divisions: F64
keywords: Science & Technology, Multidisciplinary Sciences, Science & Technology - Other Topics, NADH-UBIQUINONE OXIDOREDUCTASE, IRON-SULFUR CLUSTERS, PHOTOSYSTEM-I, EPR-SPECTRA, SUPEROXIDE-PRODUCTION, GENE ORGANIZATION, CHLOROPLAST NDH, 4FE-4S CLUSTER, SUBUNIT, FLOW
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abstract: Photosynthesis and respiration rely upon a proton gradient to produce ATP. In photosynthesis, the Respiratory Complex I homologue, Photosynthetic Complex I (PS-CI) is proposed to couple ferredoxin oxidation and plastoquinone reduction to proton pumping across
thylakoid membranes. However, little is known about the PS-CI molecular mechanism and
attempts to understand its function have previously been frustrated by its large size and high
lability. Here, we overcome these challenges by pushing the limits in sample size and
spectroscopic sensitivity, to determine arguably the most important property of any electron
transport enzyme – the reduction potentials of its cofactors, in this case the iron-sulphur
clusters of PS-CI (N0, N1 and N2), and unambiguously assign them to the structure using
double electron-electron resonance. We have thus determined the bioenergetics of the
electron transfer relay and provide insight into the mechanism of PS-CI, laying the foundations for understanding of how this important bioenergetic complex functions.
date: 2021-09-10
date_type: published
publisher: NATURE PORTFOLIO
official_url: https://doi.org/10.1038/s41467-021-25527-1
oa_status: green
full_text_type: pub
language: eng
primo: open
primo_central: open_green
verified: verified_manual
elements_id: 1888807
doi: 10.1038/s41467-021-25527-1
lyricists_name: Morton, John
lyricists_id: JJLMO69
actors_name: Flynn, Bernadette
actors_id: BFFLY94
actors_role: owner
full_text_status: public
publication: Nature Communications
volume: 12
number: 1
article_number: 5387
pages: 8
citation:        Richardson, KH;    Wright, JJ;    Simenas, M;    Thiemann, J;    Esteves, AM;    McGuire, G;    Myers, WK;                     ... Roessler, MM; + view all <#>        Richardson, KH;  Wright, JJ;  Simenas, M;  Thiemann, J;  Esteves, AM;  McGuire, G;  Myers, WK;  Morton, JJL;  Hippler, M;  Nowaczyk, MM;  Hanke, GT;  Roessler, MM;   - view fewer <#>    (2021)    Functional basis of electron transport within photosynthetic complex I.                   Nature Communications , 12  (1)    , Article 5387.  10.1038/s41467-021-25527-1 <https://doi.org/10.1038/s41467-021-25527-1>.       Green open access   
 
document_url: https://discovery.ucl.ac.uk/id/eprint/10135179/1/s41467-021-25527-1.pdf