eprintid: 10133768
rev_number: 14
eprint_status: archive
userid: 608
dir: disk0/10/13/37/68
datestamp: 2021-09-02 10:35:10
lastmod: 2021-09-24 21:55:39
status_changed: 2021-09-02 10:35:10
type: article
metadata_visibility: show
creators_name: Hollósi, A
creators_name: Pászty, K
creators_name: Kellermayer, M
creators_name: Charras, G
creators_name: Varga, A
title: BRAF Modulates Stretch-Induced Intercellular Gap Formation through Localized Actin Reorganization
ispublished: pub
divisions: UCL
divisions: B04
divisions: C06
divisions: F64
keywords: actin cytoskeleton; BRAF RNAi; endothelial monolayer; intercellular gaps; mechanical stretch
note: This is an open access article distributed under the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
abstract: Mechanical forces acting on cell–cell adhesion modulate the barrier function of endothelial cells. The actively remodeled actin cytoskeleton impinges on cell–cell adhesion to counteract external forces. We applied stress on endothelial monolayers by mechanical stretch to uncover the role of BRAF in the stress-induced response. Control cells responded to external forces by organizing and stabilizing actin cables in the stretched cell junctions. This was accompanied by an increase in intercellular gap formation, which was prevented in BRAF knockdown monolayers. In the absence of BRAF, there was excess stress fiber formation due to the enhanced reorganization of actin fibers. Our findings suggest that stretch-induced intercellular gap formation, leading to a decrease in barrier function of blood vessels, can be reverted by BRAF RNAi. This is important when the endothelium experiences changes in external stresses caused by high blood pressure, leading to edema, or by immune or cancer cells in inflammation or metastasis.
date: 2021-08-02
date_type: published
official_url: https://doi.org/10.3390/ijms22168989
oa_status: green
full_text_type: pub
language: eng
primo: open
primo_central: open_green
verified: verified_manual
elements_id: 1883893
doi: 10.3390/ijms22168989
lyricists_name: Charras, Guillaume
lyricists_id: GCHAR39
actors_name: Barczynska, Patrycja
actors_id: PBARC91
actors_role: owner
full_text_status: public
publication: International Journal of Molecular Sciences
volume: 22
number: 16
article_number: 8989
citation:        Hollósi, A;    Pászty, K;    Kellermayer, M;    Charras, G;    Varga, A;      (2021)    BRAF Modulates Stretch-Induced Intercellular Gap Formation through Localized Actin Reorganization.                   International Journal of Molecular Sciences , 22  (16)    , Article 8989.  10.3390/ijms22168989 <https://doi.org/10.3390/ijms22168989>.       Green open access   
 
document_url: https://discovery.ucl.ac.uk/id/eprint/10133768/1/ijms-22-08989-v2.pdf