eprintid: 10132082
rev_number: 20
eprint_status: archive
userid: 608
dir: disk0/10/13/20/82
datestamp: 2021-08-03 08:49:54
lastmod: 2023-12-11 14:12:08
status_changed: 2021-08-03 08:49:54
type: article
metadata_visibility: show
creators_name: Bayford, R
creators_name: Damaso, R
creators_name: Neshatvar, N
creators_name: Ivanenko, Y
creators_name: Rademacher, T
creators_name: Yu, W
creators_name: Seifnaraghi, N
creators_name: Ghali, L
creators_name: Parel, N
creators_name: Roitt, I
creators_name: Nordebo, S
creators_name: Demosthenous, A
title: Locating Functionalized Gold Nanoparticles Using Electrical Impedance Tomography
ispublished: pub
divisions: UCL
divisions: B04
divisions: C05
divisions: F46
note: This version is the author accepted manuscript. For information on re-use, please refer to the publisher's terms and conditions.
abstract: Abstract Objective: An imaging device to locate functionalized nanoparticles, whereby therapeutic agents are transported from the site of administration specifically to diseased tissues, remains a challenge for pharmaceutical research. Here, we show a new method based on electrical impedance tomography (EIT) to provide images of the location of gold nanoparticles (GNPs) and the excitation of GNPs with radio frequencies (RF) to change impedance permitting an estimation of their location in cell models Methods: We have created an imaging system using quantum cluster GNPs as a contrast agent, activated with RF fields to heat the functionalized GNPs, which causes a change in impedance in the surrounding region. This change is then identified with EIT. Results: Images of impedance changes of around 804% are obtained for a sample of citrate stabilized GNPs in a solution of phosphate-buffered saline. A second quantification was carried out using colorectal cancer cells incubated with culture media, and the internalization of GNPs into the colorectal cancer cells was undertaken to compare them with the EIT images. When the cells were incubated with functionalized GNPs, the change was more apparent, approximately 402%. This change was reflected in the EIT image as the cell area was more clearly identifiable from the rest of the area. Significance: EIT can be used as a new method to locate functionalized GNPs in human cells and help in the development of GNP-based drugs in humans to improve their efficacy in the future.
date: 2022-01
date_type: published
publisher: Institute of Electrical and Electronics Engineers (IEEE)
official_url: https://doi.org/10.1109/TBME.2021.3100256
oa_status: green
full_text_type: other
language: eng
primo: open
primo_central: open_green
verified: verified_manual
elements_id: 1878766
doi: 10.1109/TBME.2021.3100256
lyricists_name: Bayford, Richard
lyricists_name: Demosthenous, Andreas
lyricists_name: Neshatvar, Nazanin
lyricists_id: RBAYF91
lyricists_id: ACDEM08
lyricists_id: NESHA66
actors_name: Demosthenous, Andreas
actors_id: ACDEM08
actors_role: owner
full_text_status: public
publication: IEEE Transactions on Biomedical Engineering
volume: 69
number: 1
pagerange: 494-502
issn: 1558-2531
citation:        Bayford, R;    Damaso, R;    Neshatvar, N;    Ivanenko, Y;    Rademacher, T;    Yu, W;    Seifnaraghi, N;                     ... Demosthenous, A; + view all <#>        Bayford, R;  Damaso, R;  Neshatvar, N;  Ivanenko, Y;  Rademacher, T;  Yu, W;  Seifnaraghi, N;  Ghali, L;  Parel, N;  Roitt, I;  Nordebo, S;  Demosthenous, A;   - view fewer <#>    (2022)    Locating Functionalized Gold Nanoparticles Using Electrical Impedance Tomography.                   IEEE Transactions on Biomedical Engineering , 69  (1)   pp. 494-502.    10.1109/TBME.2021.3100256 <https://doi.org/10.1109/TBME.2021.3100256>.       Green open access   
 
document_url: https://discovery.ucl.ac.uk/id/eprint/10132082/1/Locating%20Functionalized%20Gold%20Nanoparticles%20Using%20Electrical%20Impedance%20Tomography.pdf