eprintid: 10131026 rev_number: 14 eprint_status: archive userid: 608 dir: disk0/10/13/10/26 datestamp: 2021-07-12 11:47:27 lastmod: 2021-10-02 22:08:43 status_changed: 2021-07-12 11:47:27 type: article metadata_visibility: show creators_name: Schwenke, J creators_name: Yusuf, M creators_name: Shemilt, LA creators_name: Wagner, U creators_name: Sajid, A creators_name: Morrison, GR creators_name: Zhang, F creators_name: Parsons, A creators_name: Rau, C creators_name: Robinson, IK title: Quantitative phase measurements of human cell nuclei using X-ray ptychography ispublished: pub divisions: UCL divisions: B04 divisions: C06 divisions: F64 keywords: X-ray, nuclei, ptychography, quantitative phase imaging, synchrotron note: This version is the author accepted manuscript. For information on re-use, please refer to the publisher's terms and conditions. abstract: The human cell nucleus serves as an important organelle holding the genetic blueprint for life. In this work, X-ray ptychography was applied to assess the masses of human cell nuclei using its unique phase shift information. Measurements were carried out at the I13-1 beamline at the Diamond Light Source that has extremely large transverse coherence properties. The ptychographic diffractive imaging approach allowed imaging of large structures that gave quantitative measurements of the phase shift in 2D projections. In this paper a modified ptychography algorithm that improves the quality of the reconstruction for weak scattering samples is presented. The application of this approach to calculate the mass of several human nuclei is also demonstrated. date: 2021-07 date_type: published official_url: https://doi.org/10.1107/S1600577521004586 oa_status: green full_text_type: other language: eng primo: open primo_central: open_green verified: verified_manual elements_id: 1874747 doi: 10.1107/S1600577521004586 pii: S1600577521004586 lyricists_name: Robinson, Ian lyricists_id: IKROB22 actors_name: Robinson, Ian actors_id: IKROB22 actors_role: owner full_text_status: public publication: Journal of Synchrotron Radiation volume: 28 number: 4 pagerange: 1166-1173 event_location: United States citation: Schwenke, J; Yusuf, M; Shemilt, LA; Wagner, U; Sajid, A; Morrison, GR; Zhang, F; ... Robinson, IK; + view all <#> Schwenke, J; Yusuf, M; Shemilt, LA; Wagner, U; Sajid, A; Morrison, GR; Zhang, F; Parsons, A; Rau, C; Robinson, IK; - view fewer <#> (2021) Quantitative phase measurements of human cell nuclei using X-ray ptychography. Journal of Synchrotron Radiation , 28 (4) pp. 1166-1173. 10.1107/S1600577521004586 <https://doi.org/10.1107/S1600577521004586>. Green open access document_url: https://discovery.ucl.ac.uk/id/eprint/10131026/1/Jorg_Manuscript_final2.pdf