eprintid: 10131026
rev_number: 14
eprint_status: archive
userid: 608
dir: disk0/10/13/10/26
datestamp: 2021-07-12 11:47:27
lastmod: 2021-10-02 22:08:43
status_changed: 2021-07-12 11:47:27
type: article
metadata_visibility: show
creators_name: Schwenke, J
creators_name: Yusuf, M
creators_name: Shemilt, LA
creators_name: Wagner, U
creators_name: Sajid, A
creators_name: Morrison, GR
creators_name: Zhang, F
creators_name: Parsons, A
creators_name: Rau, C
creators_name: Robinson, IK
title: Quantitative phase measurements of human cell nuclei using X-ray ptychography
ispublished: pub
divisions: UCL
divisions: B04
divisions: C06
divisions: F64
keywords: X-ray, nuclei, ptychography, quantitative phase imaging, synchrotron
note: This version is the author accepted manuscript. For information on re-use, please refer to the publisher's terms and conditions.
abstract: The human cell nucleus serves as an important organelle holding the genetic blueprint for life. In this work, X-ray ptychography was applied to assess the masses of human cell nuclei using its unique phase shift information. Measurements were carried out at the I13-1 beamline at the Diamond Light Source that has extremely large transverse coherence properties. The ptychographic diffractive imaging approach allowed imaging of large structures that gave quantitative measurements of the phase shift in 2D projections. In this paper a modified ptychography algorithm that improves the quality of the reconstruction for weak scattering samples is presented. The application of this approach to calculate the mass of several human nuclei is also demonstrated.
date: 2021-07
date_type: published
official_url: https://doi.org/10.1107/S1600577521004586
oa_status: green
full_text_type: other
language: eng
primo: open
primo_central: open_green
verified: verified_manual
elements_id: 1874747
doi: 10.1107/S1600577521004586
pii: S1600577521004586
lyricists_name: Robinson, Ian
lyricists_id: IKROB22
actors_name: Robinson, Ian
actors_id: IKROB22
actors_role: owner
full_text_status: public
publication: Journal of Synchrotron Radiation
volume: 28
number: 4
pagerange: 1166-1173
event_location: United States
citation:        Schwenke, J;    Yusuf, M;    Shemilt, LA;    Wagner, U;    Sajid, A;    Morrison, GR;    Zhang, F;             ... Robinson, IK; + view all <#>        Schwenke, J;  Yusuf, M;  Shemilt, LA;  Wagner, U;  Sajid, A;  Morrison, GR;  Zhang, F;  Parsons, A;  Rau, C;  Robinson, IK;   - view fewer <#>    (2021)    Quantitative phase measurements of human cell nuclei using X-ray ptychography.                   Journal of Synchrotron Radiation , 28  (4)   pp. 1166-1173.    10.1107/S1600577521004586 <https://doi.org/10.1107/S1600577521004586>.       Green open access   
 
document_url: https://discovery.ucl.ac.uk/id/eprint/10131026/1/Jorg_Manuscript_final2.pdf