eprintid: 10130651 rev_number: 14 eprint_status: archive userid: 608 dir: disk0/10/13/06/51 datestamp: 2021-07-05 12:47:22 lastmod: 2021-11-17 23:40:23 status_changed: 2021-07-05 12:47:22 type: article metadata_visibility: show creators_name: von Essen, M creators_name: Leung, WTM creators_name: Bosch, J creators_name: Pooley, S creators_name: Ayres, C creators_name: Price, SJ title: High pathogen prevalence in an amphibian and reptile assemblage at a site with risk factors for dispersal in Galicia, Spain ispublished: pub divisions: UCL divisions: B02 divisions: C08 note: © 2020 von Essen et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/). abstract: Ranaviruses are agents of disease, mortality and population declines in ectothermic vertebrates and emergences have been repeatedly linked to human activities. Ranaviruses in the common midwife toad ranavirus lineage are emerging in Europe. They are known to be severe multi-host pathogens of amphibians and can also cause disease in reptiles. Recurrent outbreaks of ranavirus disease and mortality affecting three species have occurred at a small reservoir in north-west Spain but no data were available on occurrence of the pathogen in the other amphibian and reptile species present or at adjacent sites. We sampled nine species of amphibians and reptiles at the reservoir and nearby sites and screened for ranavirus presence using molecular methods. Our results show infection with ranavirus in all nine species, including first reports for Hyla molleri, Pelophylax perezi, Rana iberica, and Podarcis bocagei. We detected ranavirus in all four local sites and confirmed mass mortality incidents involving Lissotriton boscai and Triturus marmoratus were ongoing. The reservoir regularly hosts water sports tournaments and the risks of ranavirus dispersal through the translocation of contaminated equipment are discussed. date: 2020 publisher: Public Library of Science (PLoS) official_url: https://doi.org/10.1371/journal.pone.0236803 oa_status: green full_text_type: pub language: eng primo: open primo_central: open_green verified: verified_manual elements_id: 1873981 doi: 10.1371/journal.pone.0236803 lyricists_name: Price, Stephen lyricists_id: SJPRI42 actors_name: Price, Stephen actors_name: Flynn, Bernadette actors_id: SJPRI42 actors_id: BFFLY94 actors_role: owner actors_role: impersonator full_text_status: public publication: PLoS One volume: 15 number: 7 article_number: e0236803 citation: von Essen, M; Leung, WTM; Bosch, J; Pooley, S; Ayres, C; Price, SJ; (2020) High pathogen prevalence in an amphibian and reptile assemblage at a site with risk factors for dispersal in Galicia, Spain. PLoS One , 15 (7) , Article e0236803. 10.1371/journal.pone.0236803 <https://doi.org/10.1371/journal.pone.0236803>. Green open access document_url: https://discovery.ucl.ac.uk/id/eprint/10130651/1/pone.0236803.pdf