eprintid: 10130389
rev_number: 14
eprint_status: archive
userid: 608
dir: disk0/10/13/03/89
datestamp: 2021-07-01 15:44:23
lastmod: 2021-09-17 22:53:10
status_changed: 2021-07-01 15:44:23
type: article
metadata_visibility: show
creators_name: Ashton, NJ
creators_name: Suárez-Calvet, M
creators_name: Karikari, TK
creators_name: Lantero-Rodriguez, J
creators_name: Snellman, A
creators_name: Sauer, M
creators_name: Simrén, J
creators_name: Minguillon, C
creators_name: Fauria, K
creators_name: Blennow, K
creators_name: Zetterberg, H
title: Effects of pre-analytical procedures on blood biomarkers for Alzheimer's pathophysiology, glial activation, and neurodegeneration.
ispublished: pub
divisions: UCL
divisions: B02
divisions: C07
divisions: D07
divisions: F86
keywords: amyloid beta, biomarkers, blood, citrate, ethylenediaminetetraacetic acid, glial fibrillary acidic protein, lithium heparin, neurofilament light, phosphorylated tau, pre‐analytics, serum
note: © 2021 The Authors. Alzheimer's & Dementia: Diagnosis, Assessment & Disease Monitoring published by Wiley Periodicals, LLC on behalf of Alzheimer's Association

This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
abstract: Introduction: We tested how tube types (ethylenediaminetetraacetic acid [EDTA], serum, lithium heparin [LiHep], and citrate) and freeze-thaw cycles affect levels of blood biomarkers for Alzheimer's disease (AD) pathophysiology, glial activation, and neuronal injury. Methods: Amyloid beta (Aβ)42, Aβ40, phosphorylated tau181 (p-tau181), glial fibrillary acidic protein, total tau (t-tau), neurofilament light, and phosphorylated neurofilament heavy protein were measured using single molecule arrays. Results: LiHep demonstrated the highest mean value for all biomarkers. Tube types were highly correlated for most biomarkers (r > 0.95) but gave significantly different absolute concentrations. Weaker correlations between tube types were found for Aβ42/40 (r = 0.63-0.86) and serum t-tau (r = 0.46-0.64). Freeze-thaw cycles highly influenced levels of serum Aβ and t-tau (P < .0001), and minor decreases in EDTA Aβ40 and EDTA p-tau181 were found after freeze-thaw cycle 4 (P < .05). Discussion: The same tube type should be used in research studies on blood biomarkers. Individual concentration cut-offs are needed for each tube type in all tested biomarkers despite being highly correlated. Serum should be avoided for Aβ42, Aβ40, and t-tau. Freeze-thaw cycles > 3 should be avoided for p-tau181.
date: 2021-06-02
date_type: published
official_url: https://doi.org/10.1002/dad2.12168
oa_status: green
full_text_type: pub
pmcid: PMC8171159
language: eng
primo: open
primo_central: open_green
verified: verified_manual
elements_id: 1872633
doi: 10.1002/dad2.12168
pii: DAD212168
lyricists_name: Zetterberg, Henrik
lyricists_id: HZETT94
actors_name: Flynn, Bernadette
actors_id: BFFLY94
actors_role: owner
full_text_status: public
publication: Alzheimer's & Dementia: Diagnosis, Assessment & Disease Monitoring
volume: 13
number: 1
article_number: e12168
event_location: United States
citation:        Ashton, NJ;    Suárez-Calvet, M;    Karikari, TK;    Lantero-Rodriguez, J;    Snellman, A;    Sauer, M;    Simrén, J;                 ... Zetterberg, H; + view all <#>        Ashton, NJ;  Suárez-Calvet, M;  Karikari, TK;  Lantero-Rodriguez, J;  Snellman, A;  Sauer, M;  Simrén, J;  Minguillon, C;  Fauria, K;  Blennow, K;  Zetterberg, H;   - view fewer <#>    (2021)    Effects of pre-analytical procedures on blood biomarkers for Alzheimer's pathophysiology, glial activation, and neurodegeneration.                   Alzheimer's & Dementia: Diagnosis, Assessment & Disease Monitoring , 13  (1)    , Article e12168.  10.1002/dad2.12168 <https://doi.org/10.1002/dad2.12168>.       Green open access   
 
document_url: https://discovery.ucl.ac.uk/id/eprint/10130389/1/dad2.12168.pdf