eprintid: 10129328
rev_number: 14
eprint_status: archive
userid: 608
dir: disk0/10/12/93/28
datestamp: 2021-06-10 09:51:00
lastmod: 2021-09-17 22:53:48
status_changed: 2021-06-10 09:51:00
type: article
metadata_visibility: show
creators_name: Altmann, P
creators_name: Ponleitner, M
creators_name: Rommer, PS
creators_name: Haslacher, H
creators_name: Mucher, P
creators_name: Leutmezer, F
creators_name: Petzold, A
creators_name: Wotawa, C
creators_name: Lanzenberger, R
creators_name: Berger, T
creators_name: Zetterberg, H
creators_name: Bsteh, G
title: Seven day pre-analytical stability of serum and plasma neurofilament light chain.
ispublished: pub
divisions: UCL
divisions: B02
divisions: C07
divisions: D07
divisions: F86
note: This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made
abstract: Neurofilament light chain (NfL) has emerged as a biomarker of neuroaxonal damage in several neurologic conditions. With increasing availability of fourth-generation immunoassays detecting NfL in blood, aspects of pre-analytical stability of this biomarker remain unanswered. This study investigated NfL concentrations in serum and plasma samples of 32 patients with neurological diagnoses using state of the art Simoa technology. We tested the effect of delayed freezing of up to 7 days and statistically determined stability and validity of measured concentrations. We found concentrations of NfL in serum and plasma to remain stable at room temperature when processing of samples is delayed up to 7 days (serum: mean absolute difference 0.9 pg/mL, intraindividual variation 1.2%; plasma: mean absolute difference 0.5 pg/mL, intraindividual variation 1.3%). Consistency of these results was nearly perfect for serum and excellent for plasma (intraclass correlation coefficients 0.99 and 0.94, respectively). In conclusion, the soluble serum and plasma NfL concentration remains stable when unprocessed blood samples are stored up to 7 days at room temperature. This information is essential for ensuring reliable study protocols, for example, when shipment of fresh samples is needed.
date: 2021-05-26
date_type: published
official_url: https://doi.org/10.1038/s41598-021-90639-z
oa_status: green
full_text_type: pub
pmcid: PMC8154890
language: eng
primo: open
primo_central: open_green
verified: verified_manual
elements_id: 1869172
doi: 10.1038/s41598-021-90639-z
pii: 10.1038/s41598-021-90639-z
lyricists_name: Petzold, Axel
lyricists_name: Zetterberg, Henrik
lyricists_id: APETZ95
lyricists_id: HZETT94
actors_name: Barczynska, Patrycja
actors_id: PBARC91
actors_role: owner
full_text_status: public
publication: Scientific Reports
volume: 11
number: 1
article_number: 11034
event_location: England
citation:        Altmann, P;    Ponleitner, M;    Rommer, PS;    Haslacher, H;    Mucher, P;    Leutmezer, F;    Petzold, A;                     ... Bsteh, G; + view all <#>        Altmann, P;  Ponleitner, M;  Rommer, PS;  Haslacher, H;  Mucher, P;  Leutmezer, F;  Petzold, A;  Wotawa, C;  Lanzenberger, R;  Berger, T;  Zetterberg, H;  Bsteh, G;   - view fewer <#>    (2021)    Seven day pre-analytical stability of serum and plasma neurofilament light chain.                   Scientific Reports , 11  (1)    , Article 11034.  10.1038/s41598-021-90639-z <https://doi.org/10.1038/s41598-021-90639-z>.       Green open access   
 
document_url: https://discovery.ucl.ac.uk/id/eprint/10129328/1/Petzold_s41598-021-90639-z.pdf