eprintid: 10129248
rev_number: 14
eprint_status: archive
userid: 608
dir: disk0/10/12/92/48
datestamp: 2021-06-07 16:21:27
lastmod: 2021-10-15 23:02:22
status_changed: 2021-06-07 16:21:27
type: article
metadata_visibility: show
creators_name: Kim, N
creators_name: Kim, E
creators_name: Kim, H
creators_name: Thomas, MR
creators_name: Najer, A
creators_name: Stevens, MM
title: Tumor-Targeting Cholesterol-Decorated DNA Nanoflowers for Intracellular Ratiometric Aptasensing
ispublished: pub
divisions: UCL
divisions: B04
divisions: C06
divisions: F64
note: © 2021 The Authors. This is an open access article under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
abstract: Probing endogenous molecular profiles is of fundamental importance to understand cellular function and processes. Despite the promise of programmable nucleic-acid-based aptasensors across the breadth of biomolecular detection, target-responsive aptasensors enabling intracellular detection are as of yet infrequently realized. Several challenges remain, including the difficulties in quantification/normalization of quencher-based intensiometric signals, stability issues of the probe architecture, and complex sensor operations often necessitating extensive structural modeling. Here, the biomimetic crystallization-empowered self-assembly of a tumor-targetable DNA–inorganic hybrid nanocomposite aptasensor is presented, which enables Förster resonance energy transfer (FRET)-based quantitative interpretation of changes in the cellular target abundance. Leveraging the design programmability and high-throughput fabrication of rolling circle amplification-driven DNA nanoarchitecture, this designer platform offers a method to self-assemble a robust nanosensor from a multifunctionality-encoded template that includes a cell-targeting aptamer, a ratiometric aptasensor, and a cholesterol-decorating element. Taking prostate cancer cells and intracellular adenosine triphosphate molecules as a model system, a synergistic effect in the targeted delivery by cholesterol and aptamers, and the feasibility of quantitative intracellular aptasensing are demonstrated. It is envisioned that this approach provides a highly generalizable strategy across wide-ranging target systems toward a biologically deliverable nanosensor that enables quantitative monitoring of the abundance of endogenous biomolecules.
date: 2021-03-18
date_type: published
publisher: WILEY-V C H VERLAG GMBH
official_url: https://doi.org/10.1002/adma.202007738
oa_status: green
full_text_type: pub
primo: open
primo_central: open_green
article_type_text: Article
verified: verified_manual
elements_id: 1846697
doi: 10.1002/adma.202007738
language_elements: English
lyricists_name: Thomas, Michael
lyricists_id: MTHOA12
actors_name: Flynn, Bernadette
actors_id: BFFLY94
actors_role: owner
full_text_status: public
publication: ADVANCED MATERIALS
volume: 33
number: 11
article_number: 2007738
pages: 10
issn: 1521-4095
citation:        Kim, N;    Kim, E;    Kim, H;    Thomas, MR;    Najer, A;    Stevens, MM;      (2021)    Tumor-Targeting Cholesterol-Decorated DNA Nanoflowers for Intracellular Ratiometric Aptasensing.                   ADVANCED MATERIALS , 33  (11)    , Article 2007738.  10.1002/adma.202007738 <https://doi.org/10.1002/adma.202007738>.       Green open access   
 
document_url: https://discovery.ucl.ac.uk/id/eprint/10129248/1/adma.202007738.pdf