<> <http://www.w3.org/2000/01/rdf-schema#comment> "The repository administrator has not yet configured an RDF license."^^<http://www.w3.org/2001/XMLSchema#string> . <> <http://xmlns.com/foaf/0.1/primaryTopic> <https://discovery.ucl.ac.uk/id/eprint/10129248> . <https://discovery.ucl.ac.uk/id/eprint/10129248> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://purl.org/ontology/bibo/AcademicArticle> . <https://discovery.ucl.ac.uk/id/eprint/10129248> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://purl.org/ontology/bibo/Article> . <https://discovery.ucl.ac.uk/id/eprint/10129248> <http://purl.org/dc/terms/title> "Tumor-Targeting Cholesterol-Decorated DNA Nanoflowers for Intracellular Ratiometric Aptasensing"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/eprint/10129248> <http://purl.org/ontology/bibo/abstract> "Probing endogenous molecular profiles is of fundamental importance to understand cellular function and processes. Despite the promise of programmable nucleic-acid-based aptasensors across the breadth of biomolecular detection, target-responsive aptasensors enabling intracellular detection are as of yet infrequently realized. Several challenges remain, including the difficulties in quantification/normalization of quencher-based intensiometric signals, stability issues of the probe architecture, and complex sensor operations often necessitating extensive structural modeling. Here, the biomimetic crystallization-empowered self-assembly of a tumor-targetable DNA–inorganic hybrid nanocomposite aptasensor is presented, which enables Förster resonance energy transfer (FRET)-based quantitative interpretation of changes in the cellular target abundance. Leveraging the design programmability and high-throughput fabrication of rolling circle amplification-driven DNA nanoarchitecture, this designer platform offers a method to self-assemble a robust nanosensor from a multifunctionality-encoded template that includes a cell-targeting aptamer, a ratiometric aptasensor, and a cholesterol-decorating element. Taking prostate cancer cells and intracellular adenosine triphosphate molecules as a model system, a synergistic effect in the targeted delivery by cholesterol and aptamers, and the feasibility of quantitative intracellular aptasensing are demonstrated. It is envisioned that this approach provides a highly generalizable strategy across wide-ranging target systems toward a biologically deliverable nanosensor that enables quantitative monitoring of the abundance of endogenous biomolecules."^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/eprint/10129248> <http://purl.org/dc/terms/date> "2021-03-18" . <https://discovery.ucl.ac.uk/id/document/1325453> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://purl.org/ontology/bibo/Document> . <https://discovery.ucl.ac.uk/id/eprint/10129248> <http://purl.org/ontology/bibo/volume> "33" . <https://discovery.ucl.ac.uk/id/eprint/10129248> <http://purl.org/ontology/bibo/issue> "11" . <https://discovery.ucl.ac.uk/id/org/ext-4fe7120e0936483c7dafdfc019a20a64> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://xmlns.com/foaf/0.1/Organization> . <https://discovery.ucl.ac.uk/id/org/ext-4fe7120e0936483c7dafdfc019a20a64> <http://xmlns.com/foaf/0.1/name> "WILEY-V C H VERLAG GMBH"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/eprint/10129248> <http://purl.org/dc/terms/publisher> <https://discovery.ucl.ac.uk/id/org/ext-4fe7120e0936483c7dafdfc019a20a64> . <https://discovery.ucl.ac.uk/id/publication/ext-15214095> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://purl.org/ontology/bibo/Collection> . <https://discovery.ucl.ac.uk/id/publication/ext-15214095> <http://xmlns.com/foaf/0.1/name> "ADVANCED MATERIALS"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/eprint/10129248> <http://purl.org/dc/terms/isPartOf> <https://discovery.ucl.ac.uk/id/publication/ext-15214095> . <https://discovery.ucl.ac.uk/id/publication/ext-15214095> <http://www.w3.org/2002/07/owl#sameAs> <urn:issn:15214095> . <https://discovery.ucl.ac.uk/id/publication/ext-15214095> <http://purl.org/ontology/bibo/issn> "15214095" . <https://discovery.ucl.ac.uk/id/eprint/10129248> <http://purl.org/ontology/bibo/status> <http://purl.org/ontology/bibo/status/published> . <https://discovery.ucl.ac.uk/id/eprint/10129248> <http://purl.org/dc/terms/creator> <https://discovery.ucl.ac.uk/id/person/ext-34d831a5217a9fe7bb95e43ac3de78d9> . <https://discovery.ucl.ac.uk/id/eprint/10129248> <http://purl.org/ontology/bibo/authorList> <https://discovery.ucl.ac.uk/id/eprint/10129248#authors> . <https://discovery.ucl.ac.uk/id/eprint/10129248#authors> <http://www.w3.org/1999/02/22-rdf-syntax-ns#_1> <https://discovery.ucl.ac.uk/id/person/ext-34d831a5217a9fe7bb95e43ac3de78d9> . <https://discovery.ucl.ac.uk/id/eprint/10129248> <http://purl.org/dc/terms/creator> <https://discovery.ucl.ac.uk/id/person/ext-f0b9359352b15222f1627b9474c682b8> . <https://discovery.ucl.ac.uk/id/eprint/10129248> <http://purl.org/ontology/bibo/authorList> <https://discovery.ucl.ac.uk/id/eprint/10129248#authors> . <https://discovery.ucl.ac.uk/id/eprint/10129248#authors> <http://www.w3.org/1999/02/22-rdf-syntax-ns#_2> <https://discovery.ucl.ac.uk/id/person/ext-f0b9359352b15222f1627b9474c682b8> . <https://discovery.ucl.ac.uk/id/eprint/10129248> <http://purl.org/dc/terms/creator> <https://discovery.ucl.ac.uk/id/person/ext-e2c909fa72fa263329591beeae230b69> . <https://discovery.ucl.ac.uk/id/eprint/10129248> <http://purl.org/ontology/bibo/authorList> <https://discovery.ucl.ac.uk/id/eprint/10129248#authors> . <https://discovery.ucl.ac.uk/id/eprint/10129248#authors> <http://www.w3.org/1999/02/22-rdf-syntax-ns#_3> <https://discovery.ucl.ac.uk/id/person/ext-e2c909fa72fa263329591beeae230b69> . <https://discovery.ucl.ac.uk/id/eprint/10129248> <http://purl.org/dc/terms/creator> <https://discovery.ucl.ac.uk/id/person/ext-ffee8cb290045ff4121e1b89ec44c7f4> . <https://discovery.ucl.ac.uk/id/eprint/10129248> <http://purl.org/ontology/bibo/authorList> <https://discovery.ucl.ac.uk/id/eprint/10129248#authors> . <https://discovery.ucl.ac.uk/id/eprint/10129248#authors> <http://www.w3.org/1999/02/22-rdf-syntax-ns#_4> <https://discovery.ucl.ac.uk/id/person/ext-ffee8cb290045ff4121e1b89ec44c7f4> . <https://discovery.ucl.ac.uk/id/eprint/10129248> <http://purl.org/dc/terms/creator> <https://discovery.ucl.ac.uk/id/person/ext-c67fca573cad74b43edfe3fa0697fb3a> . <https://discovery.ucl.ac.uk/id/eprint/10129248> <http://purl.org/ontology/bibo/authorList> <https://discovery.ucl.ac.uk/id/eprint/10129248#authors> . <https://discovery.ucl.ac.uk/id/eprint/10129248#authors> <http://www.w3.org/1999/02/22-rdf-syntax-ns#_5> <https://discovery.ucl.ac.uk/id/person/ext-c67fca573cad74b43edfe3fa0697fb3a> . <https://discovery.ucl.ac.uk/id/eprint/10129248> <http://purl.org/dc/terms/creator> <https://discovery.ucl.ac.uk/id/person/ext-3fe7efcae130e67fa4fc84974396b6fc> . <https://discovery.ucl.ac.uk/id/eprint/10129248> <http://purl.org/ontology/bibo/authorList> <https://discovery.ucl.ac.uk/id/eprint/10129248#authors> . <https://discovery.ucl.ac.uk/id/eprint/10129248#authors> <http://www.w3.org/1999/02/22-rdf-syntax-ns#_6> <https://discovery.ucl.ac.uk/id/person/ext-3fe7efcae130e67fa4fc84974396b6fc> . <https://discovery.ucl.ac.uk/id/person/ext-f0b9359352b15222f1627b9474c682b8> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://xmlns.com/foaf/0.1/Person> . <https://discovery.ucl.ac.uk/id/person/ext-f0b9359352b15222f1627b9474c682b8> <http://xmlns.com/foaf/0.1/givenName> "E"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/person/ext-f0b9359352b15222f1627b9474c682b8> <http://xmlns.com/foaf/0.1/familyName> "Kim"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/person/ext-f0b9359352b15222f1627b9474c682b8> <http://xmlns.com/foaf/0.1/name> "E Kim"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/person/ext-c67fca573cad74b43edfe3fa0697fb3a> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://xmlns.com/foaf/0.1/Person> . <https://discovery.ucl.ac.uk/id/person/ext-c67fca573cad74b43edfe3fa0697fb3a> <http://xmlns.com/foaf/0.1/givenName> "A"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/person/ext-c67fca573cad74b43edfe3fa0697fb3a> <http://xmlns.com/foaf/0.1/familyName> "Najer"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/person/ext-c67fca573cad74b43edfe3fa0697fb3a> <http://xmlns.com/foaf/0.1/name> "A Najer"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/person/ext-e2c909fa72fa263329591beeae230b69> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://xmlns.com/foaf/0.1/Person> . <https://discovery.ucl.ac.uk/id/person/ext-e2c909fa72fa263329591beeae230b69> <http://xmlns.com/foaf/0.1/givenName> "H"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/person/ext-e2c909fa72fa263329591beeae230b69> <http://xmlns.com/foaf/0.1/familyName> "Kim"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/person/ext-e2c909fa72fa263329591beeae230b69> <http://xmlns.com/foaf/0.1/name> "H Kim"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/person/ext-ffee8cb290045ff4121e1b89ec44c7f4> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://xmlns.com/foaf/0.1/Person> . <https://discovery.ucl.ac.uk/id/person/ext-ffee8cb290045ff4121e1b89ec44c7f4> <http://xmlns.com/foaf/0.1/givenName> "MR"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/person/ext-ffee8cb290045ff4121e1b89ec44c7f4> <http://xmlns.com/foaf/0.1/familyName> "Thomas"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/person/ext-ffee8cb290045ff4121e1b89ec44c7f4> <http://xmlns.com/foaf/0.1/name> "MR Thomas"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/person/ext-34d831a5217a9fe7bb95e43ac3de78d9> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://xmlns.com/foaf/0.1/Person> . <https://discovery.ucl.ac.uk/id/person/ext-34d831a5217a9fe7bb95e43ac3de78d9> <http://xmlns.com/foaf/0.1/givenName> "N"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/person/ext-34d831a5217a9fe7bb95e43ac3de78d9> <http://xmlns.com/foaf/0.1/familyName> "Kim"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/person/ext-34d831a5217a9fe7bb95e43ac3de78d9> <http://xmlns.com/foaf/0.1/name> "N Kim"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/person/ext-3fe7efcae130e67fa4fc84974396b6fc> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://xmlns.com/foaf/0.1/Person> . <https://discovery.ucl.ac.uk/id/person/ext-3fe7efcae130e67fa4fc84974396b6fc> <http://xmlns.com/foaf/0.1/givenName> "MM"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/person/ext-3fe7efcae130e67fa4fc84974396b6fc> <http://xmlns.com/foaf/0.1/familyName> "Stevens"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/person/ext-3fe7efcae130e67fa4fc84974396b6fc> <http://xmlns.com/foaf/0.1/name> "MM Stevens"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/eprint/10129248> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://eprints.org/ontology/EPrint> . <https://discovery.ucl.ac.uk/id/eprint/10129248> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://eprints.org/ontology/ArticleEPrint> . <https://discovery.ucl.ac.uk/id/eprint/10129248> <http://purl.org/dc/terms/isPartOf> <https://discovery.ucl.ac.uk/id/repository> . <https://discovery.ucl.ac.uk/id/eprint/10129248> <http://eprints.org/ontology/hasDocument> <https://discovery.ucl.ac.uk/id/document/1325453> . <https://discovery.ucl.ac.uk/id/document/1325453> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://eprints.org/ontology/Document> . <https://discovery.ucl.ac.uk/id/document/1325453> <http://www.w3.org/2000/01/rdf-schema#label> "Tumor-Targeting Cholesterol-Decorated DNA Nanoflowers for Intracellular Ratiometric Aptasensing (Text)"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/eprint/10129248> <http://purl.org/dc/elements/1.1/hasVersion> <https://discovery.ucl.ac.uk/id/document/1325453> . <https://discovery.ucl.ac.uk/id/eprint/10129248> <http://eprints.org/ontology/hasPublished> <https://discovery.ucl.ac.uk/id/document/1325453> . <https://discovery.ucl.ac.uk/id/document/1325453> <http://eprints.org/ontology/hasFile> <https://discovery.ucl.ac.uk/id/eprint/10129248/1/adma.202007738.pdf> . <https://discovery.ucl.ac.uk/id/document/1325453> <http://purl.org/dc/terms/hasPart> <https://discovery.ucl.ac.uk/id/eprint/10129248/1/adma.202007738.pdf> . <https://discovery.ucl.ac.uk/id/eprint/10129248/1/adma.202007738.pdf> <http://www.w3.org/2000/01/rdf-schema#label> "adma.202007738.pdf"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/eprint/10129248> <http://eprints.org/ontology/hasDocument> <https://discovery.ucl.ac.uk/id/document/1325454> . <https://discovery.ucl.ac.uk/id/document/1325454> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://eprints.org/ontology/Document> . <https://discovery.ucl.ac.uk/id/document/1325454> <http://www.w3.org/2000/01/rdf-schema#label> "Tumor-Targeting Cholesterol-Decorated DNA Nanoflowers for Intracellular Ratiometric Aptasensing (Other)"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/document/1325454> <http://eprints.org/relation/isVersionOf> <https://discovery.ucl.ac.uk/id/document/1325453> . <https://discovery.ucl.ac.uk/id/document/1325454> <http://eprints.org/relation/isVolatileVersionOf> <https://discovery.ucl.ac.uk/id/document/1325453> . <https://discovery.ucl.ac.uk/id/document/1325454> <http://eprints.org/relation/islightboxThumbnailVersionOf> <https://discovery.ucl.ac.uk/id/document/1325453> . <https://discovery.ucl.ac.uk/id/document/1325454> <http://eprints.org/ontology/hasFile> <https://discovery.ucl.ac.uk/id/eprint/10129248/2/lightbox.jpg> . 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