TY  - JOUR
TI  - The exosome journey: from biogenesis to uptake and intracellular signalling.
JF  - Cell Communication and Signaling
VL  - 19
IS  - 1
KW  - Endocytosis
KW  -  Exosomes
KW  -  Extracellular vesicles
KW  -  Intercellular communication
KW  -  Lipid rafts
KW  -  Multivesicular bodies
KW  -  Rab GTPases
KW  -  Targeting
KW  -  Tetraspanins
AV  - public
N2  - The use of exosomes in clinical settings is progressively becoming a reality, as clinical trials testing exosomes for diagnostic and therapeutic applications are generating remarkable interest from the scientific community and investors. Exosomes are small extracellular vesicles secreted by all cell types playing intercellular communication roles in health and disease by transferring cellular cargoes such as functional proteins, metabolites and nucleic acids to recipient cells. An in-depth understanding of exosome biology is therefore essential to ensure clinical development of exosome based investigational therapeutic products. Here we summarise the most up-to-date knowkedge about the complex biological journey of exosomes from biogenesis and secretion, transport and uptake to their intracellular signalling. We delineate the major pathways and molecular players that influence each step of exosome physiology, highlighting the routes of interest, which will be of benefit to exosome manipulation and engineering. We highlight the main controversies in the field of exosome research: their adequate definition, characterisation and biogenesis at plasma membrane. We also delineate the most common identified pitfalls affecting exosome research and development. Unravelling exosome physiology is key to their ultimate progression towards clinical applications. Video Abstract.
ID  - discovery10127020
UR  - https://doi.org/10.1186/s12964-021-00730-1
N1  - This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made.
Y1  - 2021/04/23/
A1  - Gurung, S
A1  - Perocheau, D
A1  - Touramanidou, L
A1  - Baruteau, J
ER  -