eprintid: 10121509 rev_number: 19 eprint_status: archive userid: 608 dir: disk0/10/12/15/09 datestamp: 2021-02-12 15:32:59 lastmod: 2022-01-29 07:10:16 status_changed: 2021-02-12 15:32:59 type: article metadata_visibility: show creators_name: Devarajan, A creators_name: Kim, Y-C creators_name: Isakovic, AF creators_name: Gater, DL title: Effect of cholecalciferol on unsaturated model membranes ispublished: pub divisions: UCL divisions: B16 divisions: B14 divisions: C31 keywords: DOPC, FTIR, NMR, Vitamin D, Vitamin D binding protein note: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions. abstract: Vitamin D plays an important role in many physiological processes, particularly calcium and phosphorous homeostasis. The biochemistry of vitamin D is also complex, encompassing a range of active molecules that may be either endogenous or dietary in origin. The role of lipids and fats in the production, processing and use of vitamin D is an interesting one, with a relative paucity of model studies into the interactions of vitamin D with lipidic systems such as micelles and vesicles. Here, we have studied the effect of vitamin D3 in simple unsaturated phospholipid systems. We used NMR and FTIR spectroscopy to investigate the effect of increasing vitamin D concentration on the structure and dynamics of the lipid chains and interfacial region. In order to link these model studies with more complex biomimetic environments, we compare results in the presence of buffer and vitamin D binding protein. We have also used DLS to determine that vitamin D3-DOPC vesicles can retain their size distribution for varying amounts of time in different conditions. We find that the acyl chain region of vitamin D3-DOPC membranes are generally disordered, and that the addition of buffer and/or protein alters the properties of the interfacial region. date: 2021-03 date_type: published publisher: Elsevier BV official_url: https://doi.org/10.1016/j.chemphyslip.2021.105058 oa_status: green full_text_type: other language: eng primo: open primo_central: open_green verified: verified_manual elements_id: 1845464 doi: 10.1016/j.chemphyslip.2021.105058 lyricists_name: Gater, Deborah lyricists_id: DLGAT20 actors_name: Allington-Smith, Dominic actors_id: DAALL44 actors_role: owner full_text_status: public publication: Chemistry and Physics of Lipids volume: 235 article_number: 105058 citation: Devarajan, A; Kim, Y-C; Isakovic, AF; Gater, DL; (2021) Effect of cholecalciferol on unsaturated model membranes. Chemistry and Physics of Lipids , 235 , Article 105058. 10.1016/j.chemphyslip.2021.105058 <https://doi.org/10.1016/j.chemphyslip.2021.105058>. Green open access document_url: https://discovery.ucl.ac.uk/id/eprint/10121509/3/Gater_VD3-DOPCpaper_FINAL%20Submitted%20Accepted.pdf