eprintid: 10120292 rev_number: 21 eprint_status: archive userid: 608 dir: disk0/10/12/02/92 datestamp: 2021-02-02 16:53:36 lastmod: 2021-10-09 22:22:40 status_changed: 2021-02-02 16:53:36 type: article metadata_visibility: show creators_name: Laha, D creators_name: Portela-Torres, P creators_name: Desfougères, Y creators_name: Saiardi, A title: Inositol phosphate kinases in the eukaryote landscape ispublished: inpress divisions: UCL divisions: B02 divisions: C08 divisions: D77 keywords: Animal, Evolution, Inositol, Kinase, Metabolism, Phytic acid, Plant, Signalling note: © 2021 The Authors. Published by Elsevier Ltd under a Creative Commons license (https://creativecommons.org/licenses/by/4.0/). abstract: Inositol phosphate encompasses a large multifaceted family of signalling molecules that originate from the combinatorial attachment of phosphate groups to the inositol ring. To date, four distinct inositol kinases have been identified, namely, IPK, ITPK, IPPK (IP5-2K), and PPIP5K. Although, ITPKs have recently been identified in archaea, eukaryotes have taken advantage of these enzymes to create a sophisticated signalling network based on inositol phosphates. However, it remains largely elusive what fundamental biochemical principles control the signalling cascade. Here, we present an evolutionary approach to understand the development of the 'inositol phosphate code' in eukaryotes. Distribution analyses of these four inositol kinase groups throughout the eukaryotic landscape reveal the loss of either ITPK, or of PPIP5K proteins in several species. Surprisingly, the loss of IPPK, an enzyme thought to catalyse the rate limiting step of IP6 (phytic acid) synthesis, was also recorded. Furthermore, this study highlights a noteworthy difference between animal (metazoan) and plant (archaeplastida) lineages. While metazoan appears to have a substantial amplification of IPK enzymes, archaeplastida genomes show a considerable increase in ITPK members. Differential evolution of IPK and ITPK between plant and animal lineage is likely reflective of converging functional adaptation of these two types of inositol kinases. Since, the IPK family comprises three sub-types IPMK, IP6K, and IP3-3K each with dedicated enzymatic specificity in metazoan, we propose that the amplified ITPK group in plant could be classified in sub-types with distinct enzymology. date: 2020-12-29 date_type: published official_url: https://doi.org/10.1016/j.jbior.2020.100782 oa_status: green full_text_type: pub language: eng primo: open primo_central: open_green verified: verified_manual elements_id: 1841897 doi: 10.1016/j.jbior.2020.100782 pii: S2212-4926(20)30093-2 lyricists_name: Saiardi, Adolfo lyricists_id: ASAIA50 actors_name: Kalinowski, Damian actors_id: DKALI47 actors_role: owner full_text_status: public publication: Advances in Biological Regulation article_number: 100782 event_location: England citation: Laha, D; Portela-Torres, P; Desfougères, Y; Saiardi, A; (2020) Inositol phosphate kinases in the eukaryote landscape. Advances in Biological Regulation , Article 100782. 10.1016/j.jbior.2020.100782 <https://doi.org/10.1016/j.jbior.2020.100782>. (In press). Green open access document_url: https://discovery.ucl.ac.uk/id/eprint/10120292/9/Saiardi_Inositol%20phosphate%20kinases%20in%20the%20eukaryote%20landscape_AOP.pdf