@article{discovery10119013,
            year = {2021},
           month = {January},
         journal = {Journal of Cell Science},
           title = {L-selectin regulates human neutrophil transendothelial migration},
            note = {This work is licensed under a Creative Commons Attribution 4.0 International License. The images
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        abstract = {The migration of circulating neutrophils towards damage/infected tissue is absolutely critical to the inflammatory response. L-selectin is a cell adhesion molecule abundantly expressed on circulating neutrophils. For over two decades, neutrophil L-selectin has been assigned the exclusive role of supporting tethering and rolling - the initial stages of the multi-step adhesion cascade. Here, we provide direct evidence for L-selectin contributing to neutrophil transendothelial migration (TEM). We show that L-selectin co-clusters with PECAM-1 - a well-characterised cell adhesion molecule involved in regulating neutrophil TEM. This co-clustering behaviour occurs specifically during TEM, which serves to augment ectodomain shedding of L-selectin and expedite the time taken for TEM (TTT) to complete. Blocking PECAM-1 signalling (through mutation of its cytoplasmic tail), PECAM-1-dependent adhesion, or L-selectin shedding, led to a significant delay in the TTT. Finally, we show that co-clustering of L-selectin with PECAM-1 occurs specifically across TNF-{\ensuremath{\alpha}}- but not IL-1{\ensuremath{\beta}}-activated endothelial monolayers - implying unique adhesion interactomes forming in a cytokine-specific manner. To our knowledge, this is the first report to implicate a non-canonical role for L-selectin in regulating neutrophil TEM.},
          author = {Rahman, I and S{\'a}nchez, AC and Davies, J and Rzeniewicz, K and Abukscem, S and Joachim, J and Green, HLH and Killock, D and Sanz, MJ and Charras, G and Parsons, M and Ivetic, A},
             url = {https://doi.org/10.1242/jcs.250340},
        keywords = {Diapedesis, JNK, PECAM-1, Transmigration, p38 MAPK}
}