TY  - JOUR
AV  - public
JF  - Biomedicines
N2  - The aim of this study was to fabricate novel self-supporting tacrolimus suppositories using semisolid extrusion 3-dimensional printing (3DP) and to investigate their efficacy in an experimental model of inflammatory bowel disease. Blends of Gelucire 44/14 and coconut oil were employed as lipid excipients to obtain suppository formulations with self-emulsifying properties, which were then tested in a TNBS (2,4,6-trinitrobenzenesulfonic acid) induced rat colitis model. Disease activity was monitored using PET/CT medical imaging; maximum standardized uptake values (SUVmax), a measure of tissue radiotracer accumulation rate, together with body weight changes and histological assessments, were used as inflammatory indices to monitor treatment efficacy. Following tacrolimus treatment, a significant reduction in SUVmax was observed on days 7 and 10 in the rat colon sections compared to non-treated animals. Histological analysis using Nancy index confirmed disease remission. Moreover, statistical analysis showed a positive correlation (R2 = 71.48%) between SUVmax values and weight changes over time. Overall, this study demonstrates the effectiveness of 3D printed tacrolimus suppositories to ameliorate colitis and highlights the utility of non-invasive PET/CT imaging to evaluate new therapies in the preclinical area.
UR  - https://doi.org/10.3390/biomedicines8120563
N1  - © 2020 by the Authors. Licensee MDPI, Basel, Switzerland. This article is an open access
article distributed under the terms and conditions of the Creative Commons Attribution
(CC BY) license (http://creativecommons.org/licenses/by/4.0/).
VL  - 8
IS  - 12
ID  - discovery10117140
TI  - 3D Printed Tacrolimus Rectal Formulations Ameliorate Colitis in an Experimental Animal Model of Inflammatory Bowel Disease
A1  - Seoane-Viaño, I
A1  - Gómez-Lado, N
A1  - Lázare-Iglesias, H
A1  - García-Otero, X
A1  - Antúnez-López, JR
A1  - Ruibal, Á
A1  - Varela-Correa, JJ
A1  - Aguiar, P
A1  - Basit, AW
A1  - Otero-Espinar, FJ
A1  - González-Barcia, M
A1  - Goyanes, A
A1  - Luzardo-Álvarez, A
A1  - Fernández-Ferreiro, A
Y1  - 2020/12//
KW  - 3D printed drug products
KW  -  M3dimaker
KW  -  PET/CT imaging
KW  -  TNBS rat model
KW  -  personalized medicines and pharmaceuticals
KW  -  rectal drug delivery
KW  -  three-dimensional printing
KW  -  ulcerative colitis
ER  -