eprintid: 10115459 rev_number: 18 eprint_status: archive userid: 608 dir: disk0/10/11/54/59 datestamp: 2020-11-22 17:57:09 lastmod: 2021-12-07 23:33:44 status_changed: 2020-11-22 17:57:09 type: article metadata_visibility: show creators_name: Simcock, IC creators_name: Hutchinson, JC creators_name: Shelmerdine, SC creators_name: Matos, JN creators_name: Sebire, NJ creators_name: Fuentes, VL creators_name: Arthurs, OJ title: Investigation of optimal sample preparation conditions with potassium triiodide and optimal imaging settings for microfocus computed tomography of excised cat hearts ispublished: pub subjects: GOSH divisions: UCL divisions: B02 divisions: D13 divisions: G26 divisions: G25 note: This version is the version of record. For information on re-use, please refer to the publisher’s terms and conditions. abstract: OBJECTIVE: To determine optimal sample preparation conditions with potassium triiodide (I2KI) and optimal imaging settings for microfocus CT (micro-CT) of excised cat hearts. SAMPLE 7 excised hearts (weight range, 10 to 17.6 g) obtained from healthy adult cats after euthanasia by IV injection of pentobarbital sodium. PROCEDURES: Following excision, the hearts were preserved in 10% formaldehyde solution. Six hearts were immersed in 1.25% I2KI solution (n = 3) or 2.5% I2KI solution (3) for a 12-day period. Micro-CT images were acquired at time 0 (prior to iodination) then approximately every 24 and 48 hours thereafter to determine optimal sample preparation conditions (ie, immersion time and concentration of I2KI solution). Identified optimal conditions were then used to prepare the seventh heart for imaging; changes in voltage, current, exposure time, and gain on image quality were evaluated to determine optimal settings (ie, maximal signal-to-noise and contrast-to-noise ratios). Images were obtained at a voxel resolution of 30 mm. A detailed morphological assessment of the main cardiac structures of the seventh heart was then performed. RESULTS: Immersion in 2.5% I2KI solution for 48 hours was optimal for sample preparation. The optimal imaging conditions included a tube voltage of 100 kV, current of 150 mA, and exposure time of 354 milliseconds; scan duration was 12 minutes. CONCLUSIONS AND CLINICAL RELEVANCE: Results provided an optimal micro-CT imaging protocol for excised cat hearts prepared with I2KI solution that could serve as a basis for future studies of micro-CT for high resolution 3-D imaging of cat hearts. date: 2020-04 date_type: published publisher: AMER VETERINARY MEDICAL ASSOC official_url: https://doi.org/10.2460/ajvr.81.4.326 oa_status: green full_text_type: pub language: eng primo: open primo_central: open_green verified: verified_manual elements_id: 1775511 doi: 10.2460/ajvr.81.4.326 lyricists_name: Arthurs, Owen lyricists_name: Sebire, Neil lyricists_name: Shelmerdine, Susan lyricists_name: Simcock, Ian lyricists_id: OARTH57 lyricists_id: NJSEB45 lyricists_id: SCSHE38 lyricists_id: ICSIM28 actors_name: Simcock, Ian actors_id: ICSIM28 actors_role: owner full_text_status: public publication: American Journal of Veterinary Research volume: 81 number: 4 pagerange: 326-333 pages: 8 citation: Simcock, IC; Hutchinson, JC; Shelmerdine, SC; Matos, JN; Sebire, NJ; Fuentes, VL; Arthurs, OJ; (2020) Investigation of optimal sample preparation conditions with potassium triiodide and optimal imaging settings for microfocus computed tomography of excised cat hearts. American Journal of Veterinary Research , 81 (4) pp. 326-333. 10.2460/ajvr.81.4.326 <https://doi.org/10.2460/ajvr.81.4.326>. Green open access document_url: https://discovery.ucl.ac.uk/id/eprint/10115459/1/Investigation%20of%20optimal%20sample%20preparation%20conditions%20with%20potassium%20triiodide%20and%20optimal%20imaging%20settings%20for%20microfocus%20computed%20tomography%20of%20excised%20cat%20hearts.pdf