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<https://discovery.ucl.ac.uk/id/eprint/10115459> <http://purl.org/dc/terms/title> "Investigation of optimal sample preparation conditions with potassium triiodide and optimal imaging settings for microfocus computed tomography of excised cat hearts"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://discovery.ucl.ac.uk/id/eprint/10115459> <http://purl.org/ontology/bibo/abstract> "OBJECTIVE:\r\nTo determine optimal sample preparation conditions with potassium triiodide (I2KI) and optimal imaging settings for microfocus CT (micro-CT) of\r\nexcised cat hearts.\r\nSAMPLE\r\n7 excised hearts (weight range, 10 to 17.6 g) obtained from healthy adult\r\ncats after euthanasia by IV injection of pentobarbital sodium.\r\nPROCEDURES:\r\nFollowing excision, the hearts were preserved in 10% formaldehyde solution. Six hearts were immersed in 1.25% I2KI solution (n = 3) or 2.5% I2KI\r\nsolution (3) for a 12-day period. Micro-CT images were acquired at time 0\r\n(prior to iodination) then approximately every 24 and 48 hours thereafter\r\nto determine optimal sample preparation conditions (ie, immersion time\r\nand concentration of I2KI solution). Identified optimal conditions were then\r\nused to prepare the seventh heart for imaging; changes in voltage, current, exposure time, and gain on image quality were evaluated to determine\r\noptimal settings (ie, maximal signal-to-noise and contrast-to-noise ratios).\r\nImages were obtained at a voxel resolution of 30 mm. A detailed morphological assessment of the main cardiac structures of the seventh heart was\r\nthen performed.\r\nRESULTS:\r\nImmersion in 2.5% I2KI solution for 48 hours was optimal for sample preparation. The optimal imaging conditions included a tube voltage of 100 kV,\r\ncurrent of 150 mA, and exposure time of 354 milliseconds; scan duration\r\nwas 12 minutes.\r\nCONCLUSIONS AND CLINICAL RELEVANCE:\r\nResults provided an optimal micro-CT imaging protocol for excised cat\r\nhearts prepared with I2KI solution that could serve as a basis for future\r\nstudies of micro-CT for high resolution 3-D imaging of cat hearts."^^<http://www.w3.org/2001/XMLSchema#string> .
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