eprintid: 10114187
rev_number: 8
eprint_status: archive
userid: 695
dir: disk0/10/11/41/87
datestamp: 2020-11-05 11:01:12
lastmod: 2020-11-05 11:01:12
status_changed: 2020-11-05 11:01:12
type: thesis
metadata_visibility: show
creators_name: Varey, Anne-Marie
title: Regulation of the immune response studied in vitro
ispublished: unpub
keywords: Health and environmental sciences; Cyclosporin
note: Thesis digitised by ProQuest.
abstract: In an attempt to study the regulation of the immune response in vitro, different agents have been used. Some appear to affect proliferation and/or differentiation of B cells and some specifically affect certain T cell subsets. Infection with pseudomonas aeruginosa is lethal m mice and the extracellular slime glycoprotein (GLP) has been identified as the pathogenic component. The effect of GLP on murine lymphocytes has been studied. GLP was shown to act like a B cell mitogen, in fact this could be described more specifically as a TI-2 polysaccharide antigen and GLP also caused non-specific terminal differentiation of B cells into immunoglobulin secretion. Repeated injections of 2'-deoxyguanosine (dGuO) can mimic purine nucleoside phosphorylase deficiency, which manifests itself as a selective T cell dysfunction. Using systems where the simultaneous development of T suppressor and cytotoxic cells occur, dGuO partially abrogates suppression but leaves the cytotoxic component unaltered. No effect on different populations of cytotoxic cells could be demonstrated and antigen presentation to T cells also appeared unaffected. The effect of cyclosporin A (CsA) on antigen presentation to T cell lines and clones was investigated. Pre-pulsing the antigen presenting cells with CsA inhibited proliferation to the specific antigen and also prevented cytokine release by these T cell lines. The effect of CsA on T cell triggering via anti-CD3 or idiotypic antibody were also studied. Conflicting results were obtained with T cell lines or clones and T cell hybridomas. Using T cell lines developed in this laboratory, the release of cytokines spontaneously, or following antigenic stimulation, were studied. Following on from this, the effect of various purified cytokines on the T-independent autologous plaque forming (PFC) assay were investigated. The majority of these factors did not affect this response with the exception of IL-5 and BCDF (SJL(4)F) which were consistently shown to have an enhancing effect in this assay. One T cell line, SJL(4), was extensively studied in order to identify the factor(s) which could enhance the autologous PFC assay. A factor produced by SJL(4) cells, SJL(4)F, appears to be distinct from IL-1, IL-2, IL-3, IL-4, IL-5, IL-6 and IFN-γ and therefore may well be a BCDF as yet undescribed in the current literature.
date: 1991
oa_status: green
full_text_type: other
thesis_class: doctoral_open
thesis_award: Ph.D
language: eng
thesis_view: UCL_Thesis
primo: open
primo_central: open_green
verified: verified_manual
full_text_status: public
pages: 288
institution: UCL (University College London)
department: Immunology
thesis_type: Doctoral
citation:        Varey, Anne-Marie;      (1991)    Regulation of the immune response studied in vitro.                   Doctoral thesis  (Ph.D), UCL (University College London).     Green open access   
 
document_url: https://discovery.ucl.ac.uk/id/eprint/10114187/1/out.pdf