eprintid: 10110398
rev_number: 19
eprint_status: archive
userid: 608
dir: disk0/10/11/03/98
datestamp: 2020-09-22 09:03:16
lastmod: 2021-09-19 22:16:30
status_changed: 2020-09-22 09:03:16
type: article
metadata_visibility: show
creators_name: Steindal, IAF
creators_name: Whitmore, D
title: Zebrafish Circadian Clock Entrainment and the Importance of Broad Spectral Light Sensitivity
ispublished: pub
divisions: UCL
divisions: B02
divisions: C08
divisions: D09
divisions: F96
keywords: zebrafish, entrainment, opsin, non-visual photopigment, circadian clock, phase shift, monochromatic light, RETINAL-PIGMENT, PINEAL, OPSIN, CELLS, GENE, EXPRESSION, PHOTORECEPTION, PARAPINOPSIN, DIVERSITY, RHYTHMS
note: © 2020 Steindal and Whitmore. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
abstract: One of the key defining features of an endogenous circadian clock is that it can be
entrained or set to local time. Though a number of cues can perform this role, light
is the predominant environmental signal that acts to entrain circadian pacemakers in
most species. For the past 20 years, a great deal of work has been performed on
the light input pathway in mammals and the role of intrinsically photosensitive retinal
ganglion cells (ipRGCs)/melanopsin in detecting and sending light information to the
suprachiasmatic nucleus (SCN). In teleost fishes, reptiles and birds, the biology of light
sensitivity is more complicated as cells and tissues can be directly light responsive.
Non-visual light signalling was described many years ago in the context of seasonal,
photoperiodic responses in birds and lizards. In the case of teleosts, in particular the
zebrafish model system, not only do peripheral tissues have a circadian pacemaker, but
possess clear, direct light sensitivity. A surprisingly wide number of opsin photopigments
have been described within these tissues, which may underpin this fundamental ability
to respond to light, though no specific functional link for any given opsin yet exists.
In this study, we show that zebrafish cells show wide spectral sensitivities, as well as
express a number of opsin photopigments – several of which are under direct clock
control. Furthermore, we also show that light outside the visual range, both ultraviolet
and infrared light, can induce clock genes in zebrafish cells. These same wavelengths
can phase shift the clock, except infrared light, which generates no shift even though
genes such as per2 and cry1a are induced.
date: 2020-08-14
date_type: published
publisher: FRONTIERS MEDIA SA
official_url: http://dx.doi.org/10.3389/fphys.2020.01002
oa_status: green
full_text_type: pub
language: eng
primo: open
primo_central: open_green
verified: verified_manual
elements_id: 1814497
doi: 10.3389/fphys.2020.01002
lyricists_name: Whitmore, David
lyricists_id: DWHIT77
actors_name: Whitmore, David
actors_id: DWHIT77
actors_role: owner
full_text_status: public
publication: Frontiers in Physiology
volume: 11
article_number: 1002
pages: 9
citation:        Steindal, IAF;    Whitmore, D;      (2020)    Zebrafish Circadian Clock Entrainment and the Importance of Broad Spectral Light Sensitivity.                   Frontiers in Physiology , 11     , Article 1002.  10.3389/fphys.2020.01002 <https://doi.org/10.3389/fphys.2020.01002>.       Green open access   
 
document_url: https://discovery.ucl.ac.uk/id/eprint/10110398/1/fphys-11-01002.pdf