<> <http://www.w3.org/2000/01/rdf-schema#comment> "The repository administrator has not yet configured an RDF license."^^<http://www.w3.org/2001/XMLSchema#string> . <> <http://xmlns.com/foaf/0.1/primaryTopic> <https://discovery.ucl.ac.uk/id/eprint/10110061> . <https://discovery.ucl.ac.uk/id/eprint/10110061> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://purl.org/ontology/bibo/AcademicArticle> . <https://discovery.ucl.ac.uk/id/eprint/10110061> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://purl.org/ontology/bibo/Article> . <https://discovery.ucl.ac.uk/id/eprint/10110061> <http://purl.org/dc/terms/title> "The transcriptional repressor Bcl6 promotes pre-TCR induced differentiation to CD4+CD8+ thymocyte and attenuates Notch1 activation"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/eprint/10110061> <http://purl.org/ontology/bibo/abstract> "Pre-TCR signal transduction is required for developing thymocytes to differentiate from CD4-CD8- double negative (DN) to CD4+CD8+ double positive (DP) cell. Notch signalling is required for T-cell fate specification and must be maintained throughout β-selection, but inappropriate Notch activation in DN4 and DP cells is oncogenic. Here, we show that pre-TCR signalling leads to increased expression of the transcriptional repressor Bcl6 and that Bcl6 is required for differentiation to DP. Conditional deletion of Bcl6 from thymocytes reduced pre-TCR-induced differentiation to DP cell, disrupted expansion and enrichment of icTCRβ+ cells within the DN population and increased DN4 cell death. It also increased Notch1 activation and Notch-mediated transcription in the DP population. Thus, Bcl6 is required in thymocyte development for efficient differentiation from DN3 to DP cell and to attenuate Notch1 activation in DP cells. Given the importance of inappropriate NOTCH1 signalling in T-ALL, and the involvement of Bcl6 in other types of leukaemia, this study is important to our understanding of T-ALL."^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/eprint/10110061> <http://purl.org/dc/terms/date> "2020-09-09" . <https://discovery.ucl.ac.uk/id/document/1175745> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://purl.org/ontology/bibo/Document> . <https://discovery.ucl.ac.uk/id/org/ext-d2596156ee130bb0a805ff1203c24d37> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://xmlns.com/foaf/0.1/Organization> . <https://discovery.ucl.ac.uk/id/org/ext-d2596156ee130bb0a805ff1203c24d37> <http://xmlns.com/foaf/0.1/name> "The Company of Biologists"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/eprint/10110061> <http://purl.org/dc/terms/publisher> <https://discovery.ucl.ac.uk/id/org/ext-d2596156ee130bb0a805ff1203c24d37> . 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<https://discovery.ucl.ac.uk/id/eprint/10110061#authors> <http://www.w3.org/1999/02/22-rdf-syntax-ns#_1> <https://discovery.ucl.ac.uk/id/person/ext-07d331b6691ad0f2c1570f915b756553> . <https://discovery.ucl.ac.uk/id/eprint/10110061> <http://purl.org/dc/terms/creator> <https://discovery.ucl.ac.uk/id/person/ext-dbb36c41e5b9dc0318334a0025458c46> . <https://discovery.ucl.ac.uk/id/eprint/10110061> <http://purl.org/ontology/bibo/authorList> <https://discovery.ucl.ac.uk/id/eprint/10110061#authors> . <https://discovery.ucl.ac.uk/id/eprint/10110061#authors> <http://www.w3.org/1999/02/22-rdf-syntax-ns#_2> <https://discovery.ucl.ac.uk/id/person/ext-dbb36c41e5b9dc0318334a0025458c46> . <https://discovery.ucl.ac.uk/id/eprint/10110061> <http://purl.org/dc/terms/creator> <https://discovery.ucl.ac.uk/id/person/ext-45fa5b418418a6efb5a4975d44c2ff22> . <https://discovery.ucl.ac.uk/id/eprint/10110061> <http://purl.org/ontology/bibo/authorList> <https://discovery.ucl.ac.uk/id/eprint/10110061#authors> . <https://discovery.ucl.ac.uk/id/eprint/10110061#authors> <http://www.w3.org/1999/02/22-rdf-syntax-ns#_3> <https://discovery.ucl.ac.uk/id/person/ext-45fa5b418418a6efb5a4975d44c2ff22> . <https://discovery.ucl.ac.uk/id/eprint/10110061> <http://purl.org/dc/terms/creator> <https://discovery.ucl.ac.uk/id/person/ext-693517aa66ca4bd9e40ee1646874939b> . <https://discovery.ucl.ac.uk/id/eprint/10110061> <http://purl.org/ontology/bibo/authorList> <https://discovery.ucl.ac.uk/id/eprint/10110061#authors> . <https://discovery.ucl.ac.uk/id/eprint/10110061#authors> <http://www.w3.org/1999/02/22-rdf-syntax-ns#_4> <https://discovery.ucl.ac.uk/id/person/ext-693517aa66ca4bd9e40ee1646874939b> . <https://discovery.ucl.ac.uk/id/eprint/10110061> <http://purl.org/dc/terms/creator> <https://discovery.ucl.ac.uk/id/person/ext-cf432fdfeb31a80c787003fa829d9c82> . <https://discovery.ucl.ac.uk/id/eprint/10110061> <http://purl.org/ontology/bibo/authorList> <https://discovery.ucl.ac.uk/id/eprint/10110061#authors> . <https://discovery.ucl.ac.uk/id/eprint/10110061#authors> <http://www.w3.org/1999/02/22-rdf-syntax-ns#_5> <https://discovery.ucl.ac.uk/id/person/ext-cf432fdfeb31a80c787003fa829d9c82> . <https://discovery.ucl.ac.uk/id/eprint/10110061> <http://purl.org/dc/terms/creator> <https://discovery.ucl.ac.uk/id/person/ext-5a285b8ee95e080fdb64b944585800c7> . <https://discovery.ucl.ac.uk/id/eprint/10110061> <http://purl.org/ontology/bibo/authorList> <https://discovery.ucl.ac.uk/id/eprint/10110061#authors> . <https://discovery.ucl.ac.uk/id/eprint/10110061#authors> <http://www.w3.org/1999/02/22-rdf-syntax-ns#_6> <https://discovery.ucl.ac.uk/id/person/ext-5a285b8ee95e080fdb64b944585800c7> . <https://discovery.ucl.ac.uk/id/eprint/10110061> <http://purl.org/dc/terms/creator> <https://discovery.ucl.ac.uk/id/person/ext-dba333ccf024d0266d0682efd09131bd> . <https://discovery.ucl.ac.uk/id/eprint/10110061> <http://purl.org/ontology/bibo/authorList> <https://discovery.ucl.ac.uk/id/eprint/10110061#authors> . <https://discovery.ucl.ac.uk/id/eprint/10110061#authors> <http://www.w3.org/1999/02/22-rdf-syntax-ns#_7> <https://discovery.ucl.ac.uk/id/person/ext-dba333ccf024d0266d0682efd09131bd> . <https://discovery.ucl.ac.uk/id/eprint/10110061> <http://purl.org/dc/terms/creator> <https://discovery.ucl.ac.uk/id/person/ext-ecb673af7cfa7689d0adf389e09202f2> . <https://discovery.ucl.ac.uk/id/eprint/10110061> <http://purl.org/ontology/bibo/authorList> <https://discovery.ucl.ac.uk/id/eprint/10110061#authors> . <https://discovery.ucl.ac.uk/id/eprint/10110061#authors> <http://www.w3.org/1999/02/22-rdf-syntax-ns#_8> <https://discovery.ucl.ac.uk/id/person/ext-ecb673af7cfa7689d0adf389e09202f2> . <https://discovery.ucl.ac.uk/id/person/ext-5a285b8ee95e080fdb64b944585800c7> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://xmlns.com/foaf/0.1/Person> . <https://discovery.ucl.ac.uk/id/person/ext-5a285b8ee95e080fdb64b944585800c7> <http://xmlns.com/foaf/0.1/givenName> "S"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/person/ext-5a285b8ee95e080fdb64b944585800c7> <http://xmlns.com/foaf/0.1/familyName> "Ross"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/person/ext-5a285b8ee95e080fdb64b944585800c7> <http://xmlns.com/foaf/0.1/name> "S Ross"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/person/ext-ecb673af7cfa7689d0adf389e09202f2> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://xmlns.com/foaf/0.1/Person> . <https://discovery.ucl.ac.uk/id/person/ext-ecb673af7cfa7689d0adf389e09202f2> <http://xmlns.com/foaf/0.1/givenName> "T"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/person/ext-ecb673af7cfa7689d0adf389e09202f2> <http://xmlns.com/foaf/0.1/familyName> "Crompton"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/person/ext-ecb673af7cfa7689d0adf389e09202f2> <http://xmlns.com/foaf/0.1/name> "T Crompton"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/person/ext-45fa5b418418a6efb5a4975d44c2ff22> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://xmlns.com/foaf/0.1/Person> . <https://discovery.ucl.ac.uk/id/person/ext-45fa5b418418a6efb5a4975d44c2ff22> <http://xmlns.com/foaf/0.1/givenName> "C-I"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/person/ext-45fa5b418418a6efb5a4975d44c2ff22> <http://xmlns.com/foaf/0.1/familyName> "Lau"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/person/ext-45fa5b418418a6efb5a4975d44c2ff22> <http://xmlns.com/foaf/0.1/name> "C-I Lau"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/person/ext-693517aa66ca4bd9e40ee1646874939b> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://xmlns.com/foaf/0.1/Person> . <https://discovery.ucl.ac.uk/id/person/ext-693517aa66ca4bd9e40ee1646874939b> <http://xmlns.com/foaf/0.1/givenName> "J"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/person/ext-693517aa66ca4bd9e40ee1646874939b> <http://xmlns.com/foaf/0.1/familyName> "Rowell"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/person/ext-693517aa66ca4bd9e40ee1646874939b> <http://xmlns.com/foaf/0.1/name> "J Rowell"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/person/ext-07d331b6691ad0f2c1570f915b756553> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://xmlns.com/foaf/0.1/Person> . <https://discovery.ucl.ac.uk/id/person/ext-07d331b6691ad0f2c1570f915b756553> <http://xmlns.com/foaf/0.1/givenName> "A"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/person/ext-07d331b6691ad0f2c1570f915b756553> <http://xmlns.com/foaf/0.1/familyName> "Solanki"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/person/ext-07d331b6691ad0f2c1570f915b756553> <http://xmlns.com/foaf/0.1/name> "A Solanki"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/person/ext-cf432fdfeb31a80c787003fa829d9c82> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://xmlns.com/foaf/0.1/Person> . <https://discovery.ucl.ac.uk/id/person/ext-cf432fdfeb31a80c787003fa829d9c82> <http://xmlns.com/foaf/0.1/givenName> "A"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/person/ext-cf432fdfeb31a80c787003fa829d9c82> <http://xmlns.com/foaf/0.1/familyName> "Barbarulo"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/person/ext-cf432fdfeb31a80c787003fa829d9c82> <http://xmlns.com/foaf/0.1/name> "A Barbarulo"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/person/ext-dba333ccf024d0266d0682efd09131bd> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://xmlns.com/foaf/0.1/Person> . <https://discovery.ucl.ac.uk/id/person/ext-dba333ccf024d0266d0682efd09131bd> <http://xmlns.com/foaf/0.1/givenName> "H"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/person/ext-dba333ccf024d0266d0682efd09131bd> <http://xmlns.com/foaf/0.1/familyName> "Sahni"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/person/ext-dba333ccf024d0266d0682efd09131bd> <http://xmlns.com/foaf/0.1/name> "H Sahni"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/person/ext-dbb36c41e5b9dc0318334a0025458c46> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://xmlns.com/foaf/0.1/Person> . <https://discovery.ucl.ac.uk/id/person/ext-dbb36c41e5b9dc0318334a0025458c46> <http://xmlns.com/foaf/0.1/givenName> "DC"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/person/ext-dbb36c41e5b9dc0318334a0025458c46> <http://xmlns.com/foaf/0.1/familyName> "Yánez"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/person/ext-dbb36c41e5b9dc0318334a0025458c46> <http://xmlns.com/foaf/0.1/name> "DC Yánez"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/eprint/10110061> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://eprints.org/ontology/EPrint> . <https://discovery.ucl.ac.uk/id/eprint/10110061> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://eprints.org/ontology/ArticleEPrint> . <https://discovery.ucl.ac.uk/id/eprint/10110061> <http://purl.org/dc/terms/isPartOf> <https://discovery.ucl.ac.uk/id/repository> . <https://discovery.ucl.ac.uk/id/eprint/10110061> <http://eprints.org/ontology/hasDocument> <https://discovery.ucl.ac.uk/id/document/1175745> . <https://discovery.ucl.ac.uk/id/document/1175745> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://eprints.org/ontology/Document> . <https://discovery.ucl.ac.uk/id/document/1175745> <http://www.w3.org/2000/01/rdf-schema#label> "The transcriptional repressor Bcl6 promotes pre-TCR induced differentiation to CD4+CD8+ thymocyte and attenuates Notch1 activation (Text)"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/eprint/10110061> <http://purl.org/dc/elements/1.1/hasVersion> <https://discovery.ucl.ac.uk/id/document/1175745> . <https://discovery.ucl.ac.uk/id/eprint/10110061> <http://eprints.org/ontology/hasAccepted> <https://discovery.ucl.ac.uk/id/document/1175745> . <https://discovery.ucl.ac.uk/id/document/1175745> <http://eprints.org/ontology/hasFile> <https://discovery.ucl.ac.uk/id/eprint/10110061/1/dev.192203.full.pdf> . <https://discovery.ucl.ac.uk/id/document/1175745> <http://purl.org/dc/terms/hasPart> <https://discovery.ucl.ac.uk/id/eprint/10110061/1/dev.192203.full.pdf> . <https://discovery.ucl.ac.uk/id/eprint/10110061/1/dev.192203.full.pdf> <http://www.w3.org/2000/01/rdf-schema#label> "dev.192203.full.pdf"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/eprint/10110061> <http://eprints.org/ontology/hasDocument> 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