eprintid: 10108756 rev_number: 18 eprint_status: archive userid: 608 dir: disk0/10/10/87/56 datestamp: 2020-09-02 11:31:45 lastmod: 2021-10-04 00:11:21 status_changed: 2020-09-02 11:31:45 type: article metadata_visibility: show creators_name: Jafaripour, SS creators_name: Gharaghani, S creators_name: Nazarshodeh, E creators_name: Haider, S creators_name: Saboury, AA title: In silico drug repositioning of FDA-approved drugs to predict new inhibitors for alpha-synuclein aggregation ispublished: pub divisions: UCL divisions: B02 divisions: C08 divisions: D10 divisions: G09 keywords: Aggregation inhibitors, Alpha-synuclein (α-syn), Pharmacophore-based repositioning note: This version is the author accepted manuscript. For information on re-use, please refer to the publisher's terms and conditions. abstract: One of the hallmarks of Parkinson's disease (PD), a long-term neurodegenerative syndrome, is the accumulation of alpha-synuclein (α-syn) fibrils. Despite numerous studies and efforts, inhibition of α-syn protein aggregation is still a challenge. To overcome this issue, we propose an in silico pharmacophore-based repositioning strategy, to find a pharmaceutical drug that, in addition to their defined role, can be used to prevent aggregation of the α-syn protein. Ligand-based pharmacophore modeling was developed and the best model was selected with validation parameters including 72 % sensitivity, 98 % specificity and goodness score about 0.7. The optimal model has three groups of hydrogen bond donor (HBD), three groups of hydrogen bond acceptor (HBA), and two aromatic rings (AR). The FDA-Approved reports in the ZINC15 database were screened with the pharmacophore model taken from inhibitor compounds. The model identified 22 hits, as promising candidate drugs for Parkinson's therapy. It is noteworthy that among these, 10 drugs have been reported to inhibition of α-syn aggregation or treat/reduce Parkinson's pathogenesis. This model was used to virtual screen ZINC, NCI databases, and natural products from the pomegranate. The results of this screen were filtered for their inability to cross the blood-brain barrier, poor oral bioavailability, etc. Finally, the selected compounds of two ZINC and NCI databases were combined and structurally clustered. Remained compounds were clustered in 28 different clusters, and the 17 compounds were introduced as final candidates. date: 2020-10 date_type: published official_url: https://doi.org/10.1016/j.compbiolchem.2020.107308 oa_status: green full_text_type: other language: eng primo: open primo_central: open_green verified: verified_manual elements_id: 1798147 doi: 10.1016/j.compbiolchem.2020.107308 pii: S1476-9271(19)31088-6 lyricists_name: Shozeb, Syed lyricists_id: SMSSH06 actors_name: Shozeb, Syed actors_id: SMSSH06 actors_role: owner full_text_status: public publication: Computational Biology and Chemistry volume: 88 article_number: 107308 event_location: England citation: Jafaripour, SS; Gharaghani, S; Nazarshodeh, E; Haider, S; Saboury, AA; (2020) In silico drug repositioning of FDA-approved drugs to predict new inhibitors for alpha-synuclein aggregation. Computational Biology and Chemistry , 88 , Article 107308. 10.1016/j.compbiolchem.2020.107308 <https://doi.org/10.1016/j.compbiolchem.2020.107308>. Green open access document_url: https://discovery.ucl.ac.uk/id/eprint/10108756/3/Shozeb_In%20silico%20drug%20repositioning%20of%20FDA-approved%20drugs%20to%20predict%20new%20inhibitors%20for%20alpha-synuclein%20aggregation_AAM.pdf