eprintid: 10107681
rev_number: 27
eprint_status: archive
userid: 608
dir: disk0/10/10/76/81
datestamp: 2020-08-13 14:54:17
lastmod: 2021-11-12 23:56:43
status_changed: 2020-09-08 10:56:28
type: article
metadata_visibility: show
creators_name: Ubbink, M
creators_name: Ben Bdira, F
creators_name: Waudby, CA
creators_name: Volkov, AN
creators_name: Schröder, SP
creators_name: Ab, E
creators_name: Codée, JDC
creators_name: Overkleeft, HS
creators_name: Aerts, HMFG
creators_name: van Ingen, H
title: Dynamics of ligand binding to a rigid glycosidase
ispublished: inpress
divisions: UCL
divisions: B02
divisions: C08
divisions: D09
divisions: G03
keywords: Glycosidases, NMR spectroscopy, dynamics, ligand binding, rigid fold
note: This is an
open access article under the terms of the Creative Commons
Attribution Non-Commercial NoDerivs License, which permits use
and distribution in any medium, provided the original work is
properly cited, the use is non-commercial, and no modifications or
adaptations are made. See here for more information: https://creativecommons.org/licenses/by-nc-nd/4.0/
abstract: The single-domain GH11 glycosidase from  Bacillus circulans  (BCX) is involved in the degradation of hemicellulose, one of the most abundant renewable biomaterials in nature. We demonstrate that BCX in solution undergoes minimal structural changes during turnover. NMR spectroscopy results show that the rigid protein matrix provides a frame for fast substrate binding in multiple conformations, accompanied by slow conversion, attributed to an enzyme induced substrate distortion. A model is proposed in which the rigid enzyme takes advantage of substrate flexibility to induce a conformation that facilitates the acyl formation step of the hydrolysis reaction.
date: 2020-09-03
date_type: published
official_url: http://dx.doi.org/10.1002/anie.202003236
oa_status: green
full_text_type: pub
language: eng
primo: open
primo_central: open_green
verified: verified_manual
elements_id: 1793800
doi: 10.1002/anie.202003236
lyricists_name: Waudby, Christopher
lyricists_id: CWAUB95
actors_name: Flynn, Bernadette
actors_id: BFFLY94
actors_role: owner
full_text_status: public
publication: Angewandte Chemie International Edition
event_location: Germany
citation:        Ubbink, M;    Ben Bdira, F;    Waudby, CA;    Volkov, AN;    Schröder, SP;    Ab, E;    Codée, JDC;             ... van Ingen, H; + view all <#>        Ubbink, M;  Ben Bdira, F;  Waudby, CA;  Volkov, AN;  Schröder, SP;  Ab, E;  Codée, JDC;  Overkleeft, HS;  Aerts, HMFG;  van Ingen, H;   - view fewer <#>    (2020)    Dynamics of ligand binding to a rigid glycosidase.                   Angewandte Chemie International Edition        10.1002/anie.202003236 <https://doi.org/10.1002/anie.202003236>.    (In press).    Green open access   
 
document_url: https://discovery.ucl.ac.uk/id/eprint/10107681/7/Waudby_anie.202003236.pdf