<> <http://www.w3.org/2000/01/rdf-schema#comment> "The repository administrator has not yet configured an RDF license."^^<http://www.w3.org/2001/XMLSchema#string> . <> <http://xmlns.com/foaf/0.1/primaryTopic> <https://discovery.ucl.ac.uk/id/eprint/10106330> . <https://discovery.ucl.ac.uk/id/eprint/10106330> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://purl.org/ontology/bibo/AcademicArticle> . <https://discovery.ucl.ac.uk/id/eprint/10106330> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://purl.org/ontology/bibo/Article> . <https://discovery.ucl.ac.uk/id/eprint/10106330> <http://purl.org/dc/terms/title> "A general strategy exploiting mâµC duplex-remodelling effect for selective detection of RNA and DNA mâµC methyltransferase activity in cells"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/eprint/10106330> <http://purl.org/ontology/bibo/abstract> "RNA:5-methylcytosine (mâµC) methyltransferases are currently the focus of intense research following a series of high-profile reports documenting their physiological links to several diseases. However, no methods exist which permit the specific analysis of RNA:mâµC methyltransferases in cells. Herein, we described how a combination of biophysical studies led us to identify distinct duplex-remodelling effects of m5C on RNA and DNA duplexes. Specifically, mâµC induces a C3′-endo to C2′-endo sugar-pucker switch in CpG RNA duplex but triggers a B-to-Z transformation in CpG DNA duplex. Inspired by these different ‘structural signatures’, we developed a mâµC-sensitive probe which fluoresces spontaneously in response to m5C-induced sugar-pucker switch, hence useful for sensing RNA:mâµC methyltransferase activity. Through the use of this probe, we achieved real-time imaging and flow cytometry analysis of NOP2/Sun RNA methyltransferase 2 (NSUN2) activity in HeLa cells. We further applied the probe to the cell-based screening of NSUN2 inhibitors. The developed strategy could also be adapted for the detection of DNA:mâµC methyltransferases. This was demonstrated by the development of DNA mâµC-probe which permits the screening of DNA methyltransferase 3A inhibitors. To our knowledge, this study represents not only the first examples of mâµC-responsive probes, but also a new strategy for discriminating RNA and DNA mâµC methyltransferase activity in cells."^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/eprint/10106330> <http://purl.org/dc/terms/date> "2020-01-10" . <https://discovery.ucl.ac.uk/id/document/1149629> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://purl.org/ontology/bibo/Document> . <https://discovery.ucl.ac.uk/id/eprint/10106330> <http://purl.org/ontology/bibo/volume> "48" . <https://discovery.ucl.ac.uk/id/eprint/10106330> <http://purl.org/ontology/bibo/issue> "1" . <https://discovery.ucl.ac.uk/id/org/ext-fef0210621b463ea6872dff71124d367> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://xmlns.com/foaf/0.1/Organization> . <https://discovery.ucl.ac.uk/id/org/ext-fef0210621b463ea6872dff71124d367> <http://xmlns.com/foaf/0.1/name> "OXFORD UNIV PRESS"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/eprint/10106330> <http://purl.org/dc/terms/publisher> <https://discovery.ucl.ac.uk/id/org/ext-fef0210621b463ea6872dff71124d367> . <https://discovery.ucl.ac.uk/id/publication/ext-03051048> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://purl.org/ontology/bibo/Collection> . <https://discovery.ucl.ac.uk/id/publication/ext-03051048> <http://xmlns.com/foaf/0.1/name> "Nucleic Acids Research"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/eprint/10106330> <http://purl.org/dc/terms/isPartOf> <https://discovery.ucl.ac.uk/id/publication/ext-03051048> . <https://discovery.ucl.ac.uk/id/publication/ext-03051048> <http://www.w3.org/2002/07/owl#sameAs> <urn:issn:03051048> . <https://discovery.ucl.ac.uk/id/publication/ext-03051048> <http://purl.org/ontology/bibo/issn> "03051048" . <https://discovery.ucl.ac.uk/id/eprint/10106330> <http://purl.org/ontology/bibo/status> <http://purl.org/ontology/bibo/status/published> . <https://discovery.ucl.ac.uk/id/eprint/10106330> <http://purl.org/dc/terms/creator> <https://discovery.ucl.ac.uk/id/person/ext-444b8c1f2034f3b3611572014eacec14> . <https://discovery.ucl.ac.uk/id/eprint/10106330> <http://purl.org/ontology/bibo/authorList> <https://discovery.ucl.ac.uk/id/eprint/10106330#authors> . <https://discovery.ucl.ac.uk/id/eprint/10106330#authors> <http://www.w3.org/1999/02/22-rdf-syntax-ns#_1> <https://discovery.ucl.ac.uk/id/person/ext-444b8c1f2034f3b3611572014eacec14> . <https://discovery.ucl.ac.uk/id/eprint/10106330> <http://purl.org/dc/terms/creator> <https://discovery.ucl.ac.uk/id/person/ext-03825a791c5c739a9b169750c87cb9f1> . <https://discovery.ucl.ac.uk/id/eprint/10106330> <http://purl.org/ontology/bibo/authorList> <https://discovery.ucl.ac.uk/id/eprint/10106330#authors> . <https://discovery.ucl.ac.uk/id/eprint/10106330#authors> <http://www.w3.org/1999/02/22-rdf-syntax-ns#_2> <https://discovery.ucl.ac.uk/id/person/ext-03825a791c5c739a9b169750c87cb9f1> . <https://discovery.ucl.ac.uk/id/eprint/10106330> <http://purl.org/dc/terms/creator> <https://discovery.ucl.ac.uk/id/person/ext-76fb666e155e6334b94ec1e2f6c0b915> . <https://discovery.ucl.ac.uk/id/eprint/10106330> <http://purl.org/ontology/bibo/authorList> <https://discovery.ucl.ac.uk/id/eprint/10106330#authors> . <https://discovery.ucl.ac.uk/id/eprint/10106330#authors> <http://www.w3.org/1999/02/22-rdf-syntax-ns#_3> <https://discovery.ucl.ac.uk/id/person/ext-76fb666e155e6334b94ec1e2f6c0b915> . <https://discovery.ucl.ac.uk/id/person/ext-03825a791c5c739a9b169750c87cb9f1> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://xmlns.com/foaf/0.1/Person> . <https://discovery.ucl.ac.uk/id/person/ext-03825a791c5c739a9b169750c87cb9f1> <http://xmlns.com/foaf/0.1/givenName> "JJA"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/person/ext-03825a791c5c739a9b169750c87cb9f1> <http://xmlns.com/foaf/0.1/familyName> "Low"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/person/ext-03825a791c5c739a9b169750c87cb9f1> <http://xmlns.com/foaf/0.1/name> "JJA Low"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/person/ext-444b8c1f2034f3b3611572014eacec14> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://xmlns.com/foaf/0.1/Person> . <https://discovery.ucl.ac.uk/id/person/ext-444b8c1f2034f3b3611572014eacec14> <http://xmlns.com/foaf/0.1/givenName> "T"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/person/ext-444b8c1f2034f3b3611572014eacec14> <http://xmlns.com/foaf/0.1/familyName> "Yang"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/person/ext-444b8c1f2034f3b3611572014eacec14> <http://xmlns.com/foaf/0.1/name> "T Yang"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/person/ext-76fb666e155e6334b94ec1e2f6c0b915> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://xmlns.com/foaf/0.1/Person> . <https://discovery.ucl.ac.uk/id/person/ext-76fb666e155e6334b94ec1e2f6c0b915> <http://xmlns.com/foaf/0.1/givenName> "ECY"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/person/ext-76fb666e155e6334b94ec1e2f6c0b915> <http://xmlns.com/foaf/0.1/familyName> "Woon"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/person/ext-76fb666e155e6334b94ec1e2f6c0b915> <http://xmlns.com/foaf/0.1/name> "ECY Woon"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/eprint/10106330> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://eprints.org/ontology/EPrint> . <https://discovery.ucl.ac.uk/id/eprint/10106330> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://eprints.org/ontology/ArticleEPrint> . <https://discovery.ucl.ac.uk/id/eprint/10106330> <http://purl.org/dc/terms/isPartOf> <https://discovery.ucl.ac.uk/id/repository> . <https://discovery.ucl.ac.uk/id/eprint/10106330> <http://eprints.org/ontology/hasDocument> <https://discovery.ucl.ac.uk/id/document/1149629> . <https://discovery.ucl.ac.uk/id/document/1149629> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://eprints.org/ontology/Document> . <https://discovery.ucl.ac.uk/id/document/1149629> <http://www.w3.org/2000/01/rdf-schema#label> "A general strategy exploiting mâµC duplex-remodelling effect for selective detection of RNA and DNA mâµC methyltransferase activity in cells (Text)"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/eprint/10106330> <http://purl.org/dc/elements/1.1/hasVersion> <https://discovery.ucl.ac.uk/id/document/1149629> . <https://discovery.ucl.ac.uk/id/eprint/10106330> <http://eprints.org/ontology/hasAccepted> <https://discovery.ucl.ac.uk/id/document/1149629> . <https://discovery.ucl.ac.uk/id/document/1149629> <http://eprints.org/ontology/hasFile> <https://discovery.ucl.ac.uk/id/eprint/10106330/1/A%20general%20strategy%20exploiting%20m5C%20duplex-remodelling%20effect%20for%20selective%20detection%20of%20RNA%20and%20DNA%20m5C%20methyltransferase%20act.pdf> . <https://discovery.ucl.ac.uk/id/document/1149629> <http://purl.org/dc/terms/hasPart> <https://discovery.ucl.ac.uk/id/eprint/10106330/1/A%20general%20strategy%20exploiting%20m5C%20duplex-remodelling%20effect%20for%20selective%20detection%20of%20RNA%20and%20DNA%20m5C%20methyltransferase%20act.pdf> . <https://discovery.ucl.ac.uk/id/eprint/10106330/1/A%20general%20strategy%20exploiting%20m5C%20duplex-remodelling%20effect%20for%20selective%20detection%20of%20RNA%20and%20DNA%20m5C%20methyltransferase%20act.pdf> <http://www.w3.org/2000/01/rdf-schema#label> "A general strategy exploiting m5C duplex-remodelling effect for selective detection of RNA and DNA m5C methyltransferase act.pdf"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/eprint/10106330> <http://eprints.org/ontology/hasDocument> <https://discovery.ucl.ac.uk/id/document/1149631> . <https://discovery.ucl.ac.uk/id/document/1149631> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://eprints.org/ontology/Document> . <https://discovery.ucl.ac.uk/id/document/1149631> <http://www.w3.org/2000/01/rdf-schema#label> "A general strategy exploiting mâµC duplex-remodelling effect for selective detection of RNA and DNA mâµC methyltransferase activity in cells (Other)"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/document/1149631> <http://eprints.org/relation/isVersionOf> <https://discovery.ucl.ac.uk/id/document/1149629> . <https://discovery.ucl.ac.uk/id/document/1149631> <http://eprints.org/relation/isVolatileVersionOf> <https://discovery.ucl.ac.uk/id/document/1149629> . <https://discovery.ucl.ac.uk/id/document/1149631> <http://eprints.org/relation/ispreviewThumbnailVersionOf> <https://discovery.ucl.ac.uk/id/document/1149629> . <https://discovery.ucl.ac.uk/id/document/1149631> <http://eprints.org/ontology/hasFile> <https://discovery.ucl.ac.uk/id/eprint/10106330/3/preview.jpg> . <https://discovery.ucl.ac.uk/id/document/1149631> <http://purl.org/dc/terms/hasPart> <https://discovery.ucl.ac.uk/id/eprint/10106330/3/preview.jpg> . <https://discovery.ucl.ac.uk/id/eprint/10106330/3/preview.jpg> <http://www.w3.org/2000/01/rdf-schema#label> "preview.jpg"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/eprint/10106330> <http://eprints.org/ontology/hasDocument> <https://discovery.ucl.ac.uk/id/document/1149632> . <https://discovery.ucl.ac.uk/id/document/1149632> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://eprints.org/ontology/Document> . <https://discovery.ucl.ac.uk/id/document/1149632> <http://www.w3.org/2000/01/rdf-schema#label> "A general strategy exploiting mâµC duplex-remodelling effect for selective detection of RNA and DNA mâµC methyltransferase activity in cells (Other)"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/document/1149632> <http://eprints.org/relation/isVersionOf> <https://discovery.ucl.ac.uk/id/document/1149629> . <https://discovery.ucl.ac.uk/id/document/1149632> <http://eprints.org/relation/isVolatileVersionOf> <https://discovery.ucl.ac.uk/id/document/1149629> . <https://discovery.ucl.ac.uk/id/document/1149632> <http://eprints.org/relation/ismediumThumbnailVersionOf> <https://discovery.ucl.ac.uk/id/document/1149629> . <https://discovery.ucl.ac.uk/id/document/1149632> <http://eprints.org/ontology/hasFile> <https://discovery.ucl.ac.uk/id/eprint/10106330/4/medium.jpg> . <https://discovery.ucl.ac.uk/id/document/1149632> <http://purl.org/dc/terms/hasPart> <https://discovery.ucl.ac.uk/id/eprint/10106330/4/medium.jpg> . <https://discovery.ucl.ac.uk/id/eprint/10106330/4/medium.jpg> <http://www.w3.org/2000/01/rdf-schema#label> "medium.jpg"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/eprint/10106330> <http://eprints.org/ontology/hasDocument> <https://discovery.ucl.ac.uk/id/document/1149633> . <https://discovery.ucl.ac.uk/id/document/1149633> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://eprints.org/ontology/Document> . <https://discovery.ucl.ac.uk/id/document/1149633> <http://www.w3.org/2000/01/rdf-schema#label> "A general strategy exploiting mâµC duplex-remodelling effect for selective detection of RNA and DNA mâµC methyltransferase activity in cells (Other)"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/document/1149633> <http://eprints.org/relation/isVersionOf> <https://discovery.ucl.ac.uk/id/document/1149629> . <https://discovery.ucl.ac.uk/id/document/1149633> <http://eprints.org/relation/isVolatileVersionOf> <https://discovery.ucl.ac.uk/id/document/1149629> . <https://discovery.ucl.ac.uk/id/document/1149633> <http://eprints.org/relation/issmallThumbnailVersionOf> 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methyltransferase activity in cells (Other)"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/document/1149634> <http://eprints.org/relation/isVersionOf> <https://discovery.ucl.ac.uk/id/document/1149629> . <https://discovery.ucl.ac.uk/id/document/1149634> <http://eprints.org/relation/isVolatileVersionOf> <https://discovery.ucl.ac.uk/id/document/1149629> . <https://discovery.ucl.ac.uk/id/document/1149634> <http://eprints.org/relation/islightboxThumbnailVersionOf> <https://discovery.ucl.ac.uk/id/document/1149629> . <https://discovery.ucl.ac.uk/id/document/1149634> <http://eprints.org/ontology/hasFile> <https://discovery.ucl.ac.uk/id/eprint/10106330/6/lightbox.jpg> . <https://discovery.ucl.ac.uk/id/document/1149634> <http://purl.org/dc/terms/hasPart> <https://discovery.ucl.ac.uk/id/eprint/10106330/6/lightbox.jpg> . <https://discovery.ucl.ac.uk/id/eprint/10106330/6/lightbox.jpg> <http://www.w3.org/2000/01/rdf-schema#label> 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