eprintid: 10105800
rev_number: 8
eprint_status: archive
userid: 695
dir: disk0/10/10/58/00
datestamp: 2020-07-21 08:52:26
lastmod: 2020-07-21 08:52:26
status_changed: 2020-07-21 08:52:26
type: thesis
metadata_visibility: show
creators_name: Flatters, Sarah Jane Louise
title: The effect of nerve injury on the spinal and peripheral actions of galanin and interleukin-6 on sensory processing
ispublished: unpub
keywords: Biological sciences; Health and environmental sciences; Nerve injury
note: Thesis digitised by ProQuest.
abstract: Neuropathic pain, subsequent to nerve damage, is a complex condition to alleviate and leads to many chronic pain patients receiving inadequate relief from their symptoms with the current treatments available. Nerve injury results in a number of anatomical, physiological and pharmacological changes. These include an upregulation of galanin, a 29-amino acid neuropepetide, and interleukin-6 (IL-6), a neuropoietic cytokine, in the dorsal root ganglion (DRG) and in the spinal cord. The aim of this thesis is to establish what role galanin and interleukin-6 play in sensory processing spinally or in the periphery in naive and spinal nerve ligated (SNL) rats. The effects of galanin and interleukin-6 were examined in vivo and in vitro using behavioural techniques and electrophysiology to record from convergent dorsal horn neurones and single nociceptive fibres. Spinal exogenous galanin profoundly inhibited neuronal responses in SNL rats, yet facilitated the same responses in naive rats. Peripheral galanin administration inhibited responses in a majority of nociceptive C-fibres and spinal neurones and facilitated responses in the remaining fibres and neurones. Following nerve injury, the proportion of neurones inhibited by galanin was increased. Spinal IL-6 administration inhibited neuronal hyperexcitability and responses to C-fibre and mechanical stimulation in SNL rats, but had no effect in naive rats. Peripheral IL-6 administration inhibited spinal neuronal responses in vivo, heat responses of nociceptive fibres in vitro and had a behavioural anti-nociceptive effect in naive rats. In SNL rats, only spinal neuronal responses to heat were inhibited by peripheral IL-6 administration. Overall these studies show that nerve injury changes the effects of galanin and interleukin-6 and suggests these systems could be potential novel targets for the treatment of neuropathic pain.
date: 2002
oa_status: green
full_text_type: other
thesis_class: doctoral_open
thesis_award: Ph.D
language: eng
thesis_view: UCL_Thesis
primo: open
primo_central: open_green
verified: verified_manual
full_text_status: public
pages: 248
institution: UCL (University College London)
department: Pharmacology
thesis_type: Doctoral
citation:        Flatters, Sarah Jane Louise;      (2002)    The effect of nerve injury on the spinal and peripheral actions of galanin and interleukin-6 on sensory processing.                   Doctoral thesis  (Ph.D), UCL (University College London).     Green open access   
 
document_url: https://discovery.ucl.ac.uk/id/eprint/10105800/1/out.pdf