eprintid: 10105363
rev_number: 15
eprint_status: archive
userid: 608
dir: disk0/10/10/53/63
datestamp: 2020-07-20 11:11:32
lastmod: 2021-10-07 22:05:02
status_changed: 2020-07-20 11:11:32
type: article
metadata_visibility: show
creators_name: Kwapong, AA
creators_name: Soares, S
creators_name: Teo, SP
creators_name: Stapleton, P
creators_name: Gibbons, S
title: Myristica lowiana Phytochemicals as Inhibitor of Plasmid Conjugation in Escherichia coli
ispublished: pub
divisions: UCL
divisions: B02
divisions: C08
divisions: D10
divisions: G09
note: Copyright © 2020 Awo Afi Kwapong et al. *is is an open access article distributed under the Creative Commons Attribution
License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is
properly cited.
abstract: Hexane extract and methanol fraction from the stem bark of Myristica lowiana specifically and significantly inhibited the conjugal transfer of the IncW plasmid R7K, a plasmid which harbors ampicillin-, streptomycin-, and spectinomycin-resistant genes. The transfer of this plasmid via the conjugative pilli of Escherichia coli was reduced by 76.5 ± 2.0% and 79.0 ± 1.2% by hexane extract and methanol fraction of M. lowiana, respectively. The hexane extract exhibited significant anti-conjugant activity at a non-cytotoxic concentration of 100 mg/L as assessed against adult human dermal fibroblast cells. The hexane extract and methanol fraction were screened using phytochemical tests, NMR spectroscopy, IR spectroscopy, and high-resolution electrospray ionization mass spectrometry (HRESIMS) and were found to contain terpenoids, sterols, and fatty acids.
date: 2020
date_type: published
official_url: http://dx.doi.org/10.1155/2020/1604638
oa_status: green
full_text_type: pub
pmcid: PMC7321511
language: eng
primo: open
primo_central: open_green
verified: verified_manual
elements_id: 1798186
doi: 10.1155/2020/1604638
lyricists_name: Stapleton, Paul
lyricists_id: PDSTA67
actors_name: Stapleton, Paul
actors_name: Harris, Jean
actors_id: PDSTA67
actors_id: JAHAR68
actors_role: owner
actors_role: impersonator
full_text_status: public
publication: Evidence-Based Complementary and Alternative Medicine
volume: 2020
article_number: 1604638
event_location: United States
issn: 1741-427X
citation:        Kwapong, AA;    Soares, S;    Teo, SP;    Stapleton, P;    Gibbons, S;      (2020)    Myristica lowiana Phytochemicals as Inhibitor of Plasmid Conjugation in Escherichia coli.                   Evidence-Based Complementary and Alternative Medicine , 2020     , Article 1604638.  10.1155/2020/1604638 <https://doi.org/10.1155/2020%2F1604638>.       Green open access   
 
document_url: https://discovery.ucl.ac.uk/id/eprint/10105363/1/1604638.pdf