TY  - JOUR
TI  - Myristica lowiana Phytochemicals as Inhibitor of Plasmid Conjugation in Escherichia coli
Y1  - 2020///
AV  - public
VL  - 2020
N1  - Copyright Š 2020 Awo Afi Kwapong et al. *is is an open access article distributed under the Creative Commons Attribution
License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is
properly cited.
ID  - discovery10105363
N2  - Hexane extract and methanol fraction from the stem bark of Myristica lowiana specifically and significantly inhibited the conjugal transfer of the IncW plasmid R7K, a plasmid which harbors ampicillin-, streptomycin-, and spectinomycin-resistant genes. The transfer of this plasmid via the conjugative pilli of Escherichia coli was reduced by 76.5?ą?2.0% and 79.0?ą?1.2% by hexane extract and methanol fraction of M. lowiana, respectively. The hexane extract exhibited significant anti-conjugant activity at a non-cytotoxic concentration of 100?mg/L as assessed against adult human dermal fibroblast cells. The hexane extract and methanol fraction were screened using phytochemical tests, NMR spectroscopy, IR spectroscopy, and high-resolution electrospray ionization mass spectrometry (HRESIMS) and were found to contain terpenoids, sterols, and fatty acids.
SN  - 1741-427X
UR  - http://dx.doi.org/10.1155/2020/1604638
JF  - Evidence-Based Complementary and Alternative Medicine
A1  - Kwapong, AA
A1  - Soares, S
A1  - Teo, SP
A1  - Stapleton, P
A1  - Gibbons, S
ER  -