%0 Journal Article
%@ 1741-427X
%A Kwapong, AA
%A Soares, S
%A Teo, SP
%A Stapleton, P
%A Gibbons, S
%D 2020
%F discovery:10105363
%J Evidence-Based Complementary and Alternative Medicine
%T Myristica lowiana Phytochemicals as Inhibitor of Plasmid Conjugation in Escherichia coli
%U https://discovery.ucl.ac.uk/id/eprint/10105363/
%V 2020
%X Hexane extract and methanol fraction from the stem bark of Myristica lowiana specifically and significantly inhibited the conjugal transfer of the IncW plasmid R7K, a plasmid which harbors ampicillin-, streptomycin-, and spectinomycin-resistant genes. The transfer of this plasmid via the conjugative pilli of Escherichia coli was reduced by 76.5 ± 2.0% and 79.0 ± 1.2% by hexane extract and methanol fraction of M. lowiana, respectively. The hexane extract exhibited significant anti-conjugant activity at a non-cytotoxic concentration of 100 mg/L as assessed against adult human dermal fibroblast cells. The hexane extract and methanol fraction were screened using phytochemical tests, NMR spectroscopy, IR spectroscopy, and high-resolution electrospray ionization mass spectrometry (HRESIMS) and were found to contain terpenoids, sterols, and fatty acids.
%Z Copyright © 2020 Awo Afi Kwapong et al. *is is an open access article distributed under the Creative Commons Attribution  License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is  properly cited.