eprintid: 10105253
rev_number: 16
eprint_status: archive
userid: 608
dir: disk0/10/10/52/53
datestamp: 2020-07-16 14:51:22
lastmod: 2021-09-17 22:51:16
status_changed: 2020-07-16 14:51:22
type: article
metadata_visibility: show
creators_name: Masunaga, A
creators_name: Liu, AW
creators_name: Tan, Y
creators_name: Scott, A
creators_name: Luscombe, NM
title: Streamlined Sampling and Cultivation of the Pelagic Cosmopolitan Larvacean, Oikopleura dioica.
ispublished: pub
divisions: UCL
divisions: B02
divisions: C08
divisions: D09
divisions: F99
note: © 2020 JoVE Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported
License
abstract: Oikopleura dioica is a planktonic chordate with exceptional filter-feeding ability, rapid generation time, conserved early development, and a compact genome. For these reasons, it is considered a useful model organism for marine ecological studies, evolutionary developmental biology, and genomics. As research often requires a steady supply of animal resources, it is useful to establish a reliable, low-maintenance culture system. Here we describe a step-by-step method for establishing an O. dioica culture. We describe how to select potential sampling sites, collection methods, target animal identification, and the set-up of the culturing system. We provide troubleshooting advice based on our own experiences. We also highlight critical factors that help sustain a robust culture system. Although the culture protocol provided here is optimized for O. dioica, we hope our sampling technique and culture setup will inspire new ideas for maintaining other fragile pelagic invertebrates.
date: 2020-06
date_type: published
official_url: https://dx.doi.org/10.3791/61279
oa_status: green
full_text_type: pub
language: eng
primo: open
primo_central: open_green
verified: verified_manual
elements_id: 1796744
doi: 10.3791/61279
lyricists_name: Luscombe, Nicholas
lyricists_id: NMLUS17
actors_name: Flynn, Bernadette
actors_id: BFFLY94
actors_role: owner
full_text_status: public
publication: Journal of Visualized Experiments
number: 160
article_number: e61279
event_location: United States
citation:        Masunaga, A;    Liu, AW;    Tan, Y;    Scott, A;    Luscombe, NM;      (2020)    Streamlined Sampling and Cultivation of the Pelagic Cosmopolitan Larvacean, Oikopleura dioica.                   Journal of Visualized Experiments   (160)    , Article e61279.  10.3791/61279 <https://doi.org/10.3791/61279>.       Green open access   
 
document_url: https://discovery.ucl.ac.uk/id/eprint/10105253/1/jove-protocol-61279-streamlined-sampling-cultivation-pelagic-cosmopolitan-larvacean.pdf