eprintid: 10105253 rev_number: 16 eprint_status: archive userid: 608 dir: disk0/10/10/52/53 datestamp: 2020-07-16 14:51:22 lastmod: 2021-09-17 22:51:16 status_changed: 2020-07-16 14:51:22 type: article metadata_visibility: show creators_name: Masunaga, A creators_name: Liu, AW creators_name: Tan, Y creators_name: Scott, A creators_name: Luscombe, NM title: Streamlined Sampling and Cultivation of the Pelagic Cosmopolitan Larvacean, Oikopleura dioica. ispublished: pub divisions: UCL divisions: B02 divisions: C08 divisions: D09 divisions: F99 note: © 2020 JoVE Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License abstract: Oikopleura dioica is a planktonic chordate with exceptional filter-feeding ability, rapid generation time, conserved early development, and a compact genome. For these reasons, it is considered a useful model organism for marine ecological studies, evolutionary developmental biology, and genomics. As research often requires a steady supply of animal resources, it is useful to establish a reliable, low-maintenance culture system. Here we describe a step-by-step method for establishing an O. dioica culture. We describe how to select potential sampling sites, collection methods, target animal identification, and the set-up of the culturing system. We provide troubleshooting advice based on our own experiences. We also highlight critical factors that help sustain a robust culture system. Although the culture protocol provided here is optimized for O. dioica, we hope our sampling technique and culture setup will inspire new ideas for maintaining other fragile pelagic invertebrates. date: 2020-06 date_type: published official_url: https://dx.doi.org/10.3791/61279 oa_status: green full_text_type: pub language: eng primo: open primo_central: open_green verified: verified_manual elements_id: 1796744 doi: 10.3791/61279 lyricists_name: Luscombe, Nicholas lyricists_id: NMLUS17 actors_name: Flynn, Bernadette actors_id: BFFLY94 actors_role: owner full_text_status: public publication: Journal of Visualized Experiments number: 160 article_number: e61279 event_location: United States citation: Masunaga, A; Liu, AW; Tan, Y; Scott, A; Luscombe, NM; (2020) Streamlined Sampling and Cultivation of the Pelagic Cosmopolitan Larvacean, Oikopleura dioica. Journal of Visualized Experiments (160) , Article e61279. 10.3791/61279 <https://doi.org/10.3791/61279>. Green open access document_url: https://discovery.ucl.ac.uk/id/eprint/10105253/1/jove-protocol-61279-streamlined-sampling-cultivation-pelagic-cosmopolitan-larvacean.pdf