TY  - INPR
TI  - Tissue Mechanics Regulate Mitotic Nuclear Dynamics during Epithelial Development
KW  - diaphanous
KW  -  interkinetic nuclear migration
KW  -  mitosis
KW  -  mitotic rounding
KW  -  pseudostratified epithelia
KW  -  rho kinase
KW  -  tissue mechanics
UR  - https://doi.org/10.1016/j.cub.2020.04.041
A1  - Kirkland, NJ
A1  - Yuen, AC
A1  - Tozluoglu, M
A1  - Hui, N
A1  - Paluch, EK
A1  - Mao, Y
Y1  - 2020/05/14/
N2  - Cell divisions are essential for tissue growth. In pseudostratified epithelia, where nuclei are staggered across
the tissue, each nucleus migrates apically before undergoing mitosis. Successful apical nuclear migration is
critical for planar-orientated cell divisions in densely packed epithelia. Most previous investigations have
focused on the local cellular mechanisms controlling nuclear migration. Inter-species and inter-organ comparisons of different pseudostratified epithelia suggest global tissue architecture may influence nuclear dynamics, but the underlying mechanisms remain elusive. Here, we use the developing Drosophila wing disc to
systematically investigate, in a single epithelial type, how changes in tissue architecture during growth influence mitotic nuclear migration. We observe distinct nuclear dynamics at discrete developmental stages, as
epithelial morphology changes. We use genetic and physical perturbations to show a direct effect of cell density on mitotic nuclear positioning. We find Rho kinase and Diaphanous, which facilitate mitotic cell rounding
in confined cell conditions, are essential for efficient apical nuclear movement. Perturbation of Diaphanous
causes increasing defects in apical nuclear migration as the tissue grows and cell density increases, and
these defects can be reversed by acute physical reduction of cell density. Our findings reveal how the mechanical environment imposed on cells within a tissue alters the molecular and cellular mechanisms adopted
by single cells for mitosis.
AV  - public
JF  - Current Biology
N1  - This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
ID  - discovery10103462
ER  -