eprintid: 10102839
rev_number: 8
eprint_status: archive
userid: 695
dir: disk0/10/10/28/39
datestamp: 2020-06-26 13:27:32
lastmod: 2020-06-26 13:27:32
status_changed: 2020-06-26 13:27:32
type: thesis
metadata_visibility: show
creators_name: Ennion, Steven James
title: A study of the myosin heavy chain gene family in the carp
ispublished: unpub
note: Thesis digitised by ProQuest.
abstract: Skeletal muscle has a striking potential for plasticity with an inherent ability to adapt to altered functional demands. The expression of various isoforms of the myosin heavy chain (MyoHC) plays a central role in facilitating these adaptive changes by conferring changes in contractile characteristics. In contrast to mammals, very little is known about the MyoHC isoforms and their genes in fish. This study aimed to characterise the family of MyoHC isogenes in the carp (Cyprinus carpio). Using genomic clone analysis and 3' RACE-PCR, the 3' untranslated regions of seven separate carp MyoHC isogenes (arbitrarily named types 1 to 7) were isolated and used to characterise the expression patterns of individual MyoHC isoforms. Northern blot analysis demonstrated that the carp MyoHC gene family is developmentally regulated and that their expression is also determined by environmental temperature. Two isoforms, types 1 and 5, are expressed in both adult and immature carp and three, types 2, 3 and 4, are expressed exclusively in immature carp. In situ hybridisation localised the expression of the type 2 MyoHC isoform to the developing pink muscle fibre layer in fry and 12 month old carp and demonstrated that distinct isoforms of the MyoHC are expressed in the red and white muscle fibres types. Types 6 and 7 MyoHC isoforms were shown to be expressed exclusively in the white muscle fibres of adult carp which had been acclimated to a warm (28°C) temperature. The expression of the type 7 MyoHC gene was localised, by in situ hybridisation, to the small diameter (10-25?m) white muscle fibres which are thought to be involved in fibre hyperplasia. The size of this gene at the genomic level was shown to be half the size of mammalian MyoHC isoforms and this difference in size was attributed to shorter introns.
date: 1994
oa_status: green
full_text_type: other
thesis_class: doctoral_open
thesis_award: Ph.D
language: eng
thesis_view: UCL_Thesis
primo: open
primo_central: open_green
verified: verified_manual
full_text_status: public
pages: 208
institution: UCL (University College London)
thesis_type: Doctoral
citation:        Ennion, Steven James;      (1994)    A study of the myosin heavy chain gene family in the carp.                   Doctoral thesis  (Ph.D), UCL (University College London).     Green open access   
 
document_url: https://discovery.ucl.ac.uk/id/eprint/10102839/1/A_study_of_the_myosin_heavy_ch.pdf