<> <http://www.w3.org/2000/01/rdf-schema#comment> "The repository administrator has not yet configured an RDF license."^^<http://www.w3.org/2001/XMLSchema#string> .
<> <http://xmlns.com/foaf/0.1/primaryTopic> <https://discovery.ucl.ac.uk/id/eprint/10100762> .
<https://discovery.ucl.ac.uk/id/eprint/10100762> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://purl.org/ontology/bibo/Thesis> .
<https://discovery.ucl.ac.uk/id/eprint/10100762> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://purl.org/ontology/bibo/Article> .
<https://discovery.ucl.ac.uk/id/eprint/10100762> <http://purl.org/dc/terms/title> "Catheptic antigen processing"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://discovery.ucl.ac.uk/id/eprint/10100762> <http://purl.org/ontology/bibo/abstract> "For an antigen to be recognized by T cells, it must first be processed into short peptide fragments and presented by an MHC molecule at the cell surface. In this thesis, the roles of cathepsins B, D and E in MHC class II antigen processing are investigated in human B cells. A vector-based antisense approach is adopted using EBV-transformed B cell presentation of tetanus toxoid to human T cell clones as a model system. A series of vectors are made containing a portion of each cathepsin gene in a sense or antisense orientation under the control of a metallothionein promoter. Stable transfection of these constructs produces a panel of EBV-cell lines which can be induced to express anti-cathepsin or control RNA. Specific reductions in cathepsin protein levels and activity is monitored in these lines using a variety of biochemical techniques. The effect of such reductions on antigen processing capacity is investigated via tetanus toxoid presentation to T cell clones. The antigen processing roles of cathepsins B, D and E are also investigated by treating B cells with short antisense oligonucleotides. Human B cells expressing the murine MHC II molecule I-Ak are pre-treated with phosphorothioate oligonucleotides and then challenged to present hen egg lysosyme (HEL) to murine T cell hybridomas. The effect of soluble cathepsin inhibitors on antigen processing is also investigated for both described antigen presentation systems, and a comparison of the three investigative approaches is made. Finally, it is shown that transcription of the cathepsin E gene is upregulated upon B cell activation (polyclonal activation of ex vivo tonsillar B cells and PMA-activation of an EBV-transformed B cell line). It is also shown that cells that do not usually present antigen (chondrosarcoma and HeLa cells) can be induced to express cathepsin E when treated with IFNγ. Mutant HeLa cells are used to unravel the potential roles of CTIIA and JAK1 in the transcriptional control mechanism."^^<http://www.w3.org/2001/XMLSchema#string> .
<https://discovery.ucl.ac.uk/id/eprint/10100762> <http://purl.org/dc/terms/date> "1997" .
<https://discovery.ucl.ac.uk/id/document/1119529> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://purl.org/ontology/bibo/Document> .
<https://discovery.ucl.ac.uk/id/org/ext-a64c3df5861c6582807add1abaadf2af> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://xmlns.com/foaf/0.1/Organization> .
<https://discovery.ucl.ac.uk/id/org/ext-a64c3df5861c6582807add1abaadf2af> <http://xmlns.com/foaf/0.1/name> "UCL (University College London)"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://discovery.ucl.ac.uk/id/eprint/10100762> <http://purl.org/dc/terms/issuer> <https://discovery.ucl.ac.uk/id/org/ext-a64c3df5861c6582807add1abaadf2af> .
<https://discovery.ucl.ac.uk/id/eprint/10100762> <http://purl.org/ontology/bibo/status> <http://purl.org/ontology/bibo/status/unpublished> .
<https://discovery.ucl.ac.uk/id/eprint/10100762> <http://purl.org/dc/terms/creator> <https://discovery.ucl.ac.uk/id/person/ext-bf479da52890e39bd92f719f90308a74> .
<https://discovery.ucl.ac.uk/id/eprint/10100762> <http://purl.org/ontology/bibo/authorList> <https://discovery.ucl.ac.uk/id/eprint/10100762#authors> .
<https://discovery.ucl.ac.uk/id/eprint/10100762#authors> <http://www.w3.org/1999/02/22-rdf-syntax-ns#_1> <https://discovery.ucl.ac.uk/id/person/ext-bf479da52890e39bd92f719f90308a74> .
<https://discovery.ucl.ac.uk/id/person/ext-bf479da52890e39bd92f719f90308a74> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://xmlns.com/foaf/0.1/Person> .
<https://discovery.ucl.ac.uk/id/person/ext-bf479da52890e39bd92f719f90308a74> <http://xmlns.com/foaf/0.1/givenName> "Louise Caroline"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://discovery.ucl.ac.uk/id/person/ext-bf479da52890e39bd92f719f90308a74> <http://xmlns.com/foaf/0.1/familyName> "Sealy"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://discovery.ucl.ac.uk/id/person/ext-bf479da52890e39bd92f719f90308a74> <http://xmlns.com/foaf/0.1/name> "Louise Caroline Sealy"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://discovery.ucl.ac.uk/id/eprint/10100762> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://eprints.org/ontology/EPrint> .
<https://discovery.ucl.ac.uk/id/eprint/10100762> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://eprints.org/ontology/ThesisEPrint> .
<https://discovery.ucl.ac.uk/id/eprint/10100762> <http://purl.org/dc/terms/isPartOf> <https://discovery.ucl.ac.uk/id/repository> .
<https://discovery.ucl.ac.uk/id/eprint/10100762> <http://eprints.org/ontology/hasDocument> <https://discovery.ucl.ac.uk/id/document/1119529> .
<https://discovery.ucl.ac.uk/id/document/1119529> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://eprints.org/ontology/Document> .
<https://discovery.ucl.ac.uk/id/document/1119529> <http://www.w3.org/2000/01/rdf-schema#label> "Catheptic antigen processing (Text)"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://discovery.ucl.ac.uk/id/document/1119529> <http://eprints.org/ontology/hasFile> <https://discovery.ucl.ac.uk/id/eprint/10100762/1/out.pdf> .
<https://discovery.ucl.ac.uk/id/document/1119529> <http://purl.org/dc/terms/hasPart> <https://discovery.ucl.ac.uk/id/eprint/10100762/1/out.pdf> .
<https://discovery.ucl.ac.uk/id/eprint/10100762/1/out.pdf> <http://www.w3.org/2000/01/rdf-schema#label> "out.pdf"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://discovery.ucl.ac.uk/id/eprint/10100762> <http://eprints.org/ontology/hasDocument> <https://discovery.ucl.ac.uk/id/document/1119546> .
<https://discovery.ucl.ac.uk/id/document/1119546> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://eprints.org/ontology/Document> .
<https://discovery.ucl.ac.uk/id/document/1119546> <http://www.w3.org/2000/01/rdf-schema#label> "Catheptic antigen processing (Other)"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://discovery.ucl.ac.uk/id/document/1119546> <http://eprints.org/relation/isVersionOf> <https://discovery.ucl.ac.uk/id/document/1119529> .
<https://discovery.ucl.ac.uk/id/document/1119546> <http://eprints.org/relation/isVolatileVersionOf> <https://discovery.ucl.ac.uk/id/document/1119529> .
<https://discovery.ucl.ac.uk/id/document/1119546> <http://eprints.org/relation/isIndexCodesVersionOf> <https://discovery.ucl.ac.uk/id/document/1119529> .
<https://discovery.ucl.ac.uk/id/document/1119546> <http://eprints.org/ontology/hasFile> <https://discovery.ucl.ac.uk/id/eprint/10100762/2/indexcodes.txt> .
<https://discovery.ucl.ac.uk/id/document/1119546> <http://purl.org/dc/terms/hasPart> <https://discovery.ucl.ac.uk/id/eprint/10100762/2/indexcodes.txt> .
<https://discovery.ucl.ac.uk/id/eprint/10100762/2/indexcodes.txt> <http://www.w3.org/2000/01/rdf-schema#label> "indexcodes.txt"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://discovery.ucl.ac.uk/id/eprint/10100762> <http://eprints.org/ontology/hasDocument> <https://discovery.ucl.ac.uk/id/document/1119547> .
<https://discovery.ucl.ac.uk/id/document/1119547> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://eprints.org/ontology/Document> .
<https://discovery.ucl.ac.uk/id/document/1119547> <http://www.w3.org/2000/01/rdf-schema#label> "Catheptic antigen processing (Other)"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://discovery.ucl.ac.uk/id/document/1119547> <http://eprints.org/relation/isVersionOf> <https://discovery.ucl.ac.uk/id/document/1119529> .
<https://discovery.ucl.ac.uk/id/document/1119547> <http://eprints.org/relation/isVolatileVersionOf> <https://discovery.ucl.ac.uk/id/document/1119529> .
<https://discovery.ucl.ac.uk/id/document/1119547> <http://eprints.org/relation/islightboxThumbnailVersionOf> <https://discovery.ucl.ac.uk/id/document/1119529> .
<https://discovery.ucl.ac.uk/id/document/1119547> <http://eprints.org/ontology/hasFile> <https://discovery.ucl.ac.uk/id/eprint/10100762/3/lightbox.jpg> .
<https://discovery.ucl.ac.uk/id/document/1119547> <http://purl.org/dc/terms/hasPart> <https://discovery.ucl.ac.uk/id/eprint/10100762/3/lightbox.jpg> .
<https://discovery.ucl.ac.uk/id/eprint/10100762/3/lightbox.jpg> <http://www.w3.org/2000/01/rdf-schema#label> "lightbox.jpg"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://discovery.ucl.ac.uk/id/eprint/10100762> <http://eprints.org/ontology/hasDocument> <https://discovery.ucl.ac.uk/id/document/1119548> .
<https://discovery.ucl.ac.uk/id/document/1119548> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://eprints.org/ontology/Document> .
<https://discovery.ucl.ac.uk/id/document/1119548> <http://www.w3.org/2000/01/rdf-schema#label> "Catheptic antigen processing (Other)"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://discovery.ucl.ac.uk/id/document/1119548> <http://eprints.org/relation/isVersionOf> <https://discovery.ucl.ac.uk/id/document/1119529> .
<https://discovery.ucl.ac.uk/id/document/1119548> <http://eprints.org/relation/isVolatileVersionOf> <https://discovery.ucl.ac.uk/id/document/1119529> .
<https://discovery.ucl.ac.uk/id/document/1119548> <http://eprints.org/relation/ispreviewThumbnailVersionOf> <https://discovery.ucl.ac.uk/id/document/1119529> .
<https://discovery.ucl.ac.uk/id/document/1119548> <http://eprints.org/ontology/hasFile> <https://discovery.ucl.ac.uk/id/eprint/10100762/4/preview.jpg> .
<https://discovery.ucl.ac.uk/id/document/1119548> <http://purl.org/dc/terms/hasPart> <https://discovery.ucl.ac.uk/id/eprint/10100762/4/preview.jpg> .
<https://discovery.ucl.ac.uk/id/eprint/10100762/4/preview.jpg> <http://www.w3.org/2000/01/rdf-schema#label> "preview.jpg"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://discovery.ucl.ac.uk/id/eprint/10100762> <http://eprints.org/ontology/hasDocument> <https://discovery.ucl.ac.uk/id/document/1119549> .
<https://discovery.ucl.ac.uk/id/document/1119549> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://eprints.org/ontology/Document> .
<https://discovery.ucl.ac.uk/id/document/1119549> <http://www.w3.org/2000/01/rdf-schema#label> "Catheptic antigen processing (Other)"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://discovery.ucl.ac.uk/id/document/1119549> <http://eprints.org/relation/isVersionOf> <https://discovery.ucl.ac.uk/id/document/1119529> .
<https://discovery.ucl.ac.uk/id/document/1119549> <http://eprints.org/relation/isVolatileVersionOf> <https://discovery.ucl.ac.uk/id/document/1119529> .
<https://discovery.ucl.ac.uk/id/document/1119549> <http://eprints.org/relation/ismediumThumbnailVersionOf> <https://discovery.ucl.ac.uk/id/document/1119529> .
<https://discovery.ucl.ac.uk/id/document/1119549> <http://eprints.org/ontology/hasFile> <https://discovery.ucl.ac.uk/id/eprint/10100762/5/medium.jpg> .
<https://discovery.ucl.ac.uk/id/document/1119549> <http://purl.org/dc/terms/hasPart> <https://discovery.ucl.ac.uk/id/eprint/10100762/5/medium.jpg> .
<https://discovery.ucl.ac.uk/id/eprint/10100762/5/medium.jpg> <http://www.w3.org/2000/01/rdf-schema#label> "medium.jpg"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://discovery.ucl.ac.uk/id/eprint/10100762> <http://eprints.org/ontology/hasDocument> <https://discovery.ucl.ac.uk/id/document/1119550> .
<https://discovery.ucl.ac.uk/id/document/1119550> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://eprints.org/ontology/Document> .
<https://discovery.ucl.ac.uk/id/document/1119550> <http://www.w3.org/2000/01/rdf-schema#label> "Catheptic antigen processing (Other)"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://discovery.ucl.ac.uk/id/document/1119550> <http://eprints.org/relation/isVersionOf> <https://discovery.ucl.ac.uk/id/document/1119529> .
<https://discovery.ucl.ac.uk/id/document/1119550> <http://eprints.org/relation/isVolatileVersionOf> <https://discovery.ucl.ac.uk/id/document/1119529> .
<https://discovery.ucl.ac.uk/id/document/1119550> <http://eprints.org/relation/issmallThumbnailVersionOf> <https://discovery.ucl.ac.uk/id/document/1119529> .
<https://discovery.ucl.ac.uk/id/document/1119550> <http://eprints.org/ontology/hasFile> <https://discovery.ucl.ac.uk/id/eprint/10100762/6/small.jpg> .
<https://discovery.ucl.ac.uk/id/document/1119550> <http://purl.org/dc/terms/hasPart> <https://discovery.ucl.ac.uk/id/eprint/10100762/6/small.jpg> .
<https://discovery.ucl.ac.uk/id/eprint/10100762/6/small.jpg> <http://www.w3.org/2000/01/rdf-schema#label> "small.jpg"^^<http://www.w3.org/2001/XMLSchema#string> .
<https://discovery.ucl.ac.uk/id/eprint/10100762> <http://www.w3.org/2000/01/rdf-schema#seeAlso> <https://discovery.ucl.ac.uk/id/eprint/10100762/> .
<https://discovery.ucl.ac.uk/id/eprint/10100762/> <http://purl.org/dc/elements/1.1/title> "HTML Summary of #10100762 \n\nCatheptic antigen processing\n\n" .
<https://discovery.ucl.ac.uk/id/eprint/10100762/> <http://purl.org/dc/elements/1.1/format> "text/html" .
<https://discovery.ucl.ac.uk/id/eprint/10100762/> <http://xmlns.com/foaf/0.1/primaryTopic> <https://discovery.ucl.ac.uk/id/eprint/10100762> .