eprintid: 10099182 rev_number: 15 eprint_status: archive userid: 608 dir: disk0/10/09/91/82 datestamp: 2020-06-01 11:18:46 lastmod: 2021-10-03 23:57:48 status_changed: 2020-06-01 11:18:46 type: article metadata_visibility: show creators_name: Emmanouilidou, E creators_name: Papagiannakis, N creators_name: Kouloulia, S creators_name: Galaziou, A creators_name: Antonellou, R creators_name: Papadimitriou, D creators_name: Athanasiadou, A creators_name: Bozi, M creators_name: Koros, C creators_name: Maniati, M creators_name: Vekrellis, K creators_name: Ioannou, PC creators_name: Stefanis, L title: Peripheral alpha-synuclein levels in patients with genetic and non-genetic forms of Parkinson's disease ispublished: pub divisions: UCL divisions: B02 divisions: C08 divisions: D77 keywords: A53T, Alpha-synuclein, Parkinson disease, Plasma, Serum note: This version is the author accepted manuscript. For information on re-use, please refer to the publisher's terms and conditions. abstract: BACKGROUND: Variations of α-synuclein levels have been reported in serum and plasma in Parkinson's Disease (PD) Patients. METHODS: Serum and plasma were obtained from PD patients without known mutations (GU-PD, n = 124)), carriers of the A53T/G209A point mutation in the α-synuclein gene (SNCA) (n = 29), and respective age-/sex-matched controls. Levels of total α-synuclein were assessed using an in-house ELISA assay. RESULTS: A statistically significant increase of α-synuclein levels was found in serum, but not plasma, from GU-PD patients compared to healthy controls. A statistically significant decrease of α-synuclein levels was found in serum and plasma from symptomatic A53T mutation carriers compared to healthy controls. Plasma α-synuclein levels were modestly negatively correlated with UPDRS part III score and disease duration in A53T-PD patients. CONCLUSION: Increased α-synuclein levels in serum of GU-PD patients suggest a systemic deregulation of α-synuclein homeostasis in PD. The opposite results in A53T-PD highlight the complexity of α-synuclein homeostatic regulation in PD, and suggest the possibility of reduced expression of the mutant allele. date: 2020-04 date_type: published official_url: https://doi.org/10.1016/j.parkreldis.2020.03.014 oa_status: green full_text_type: other language: eng primo: open primo_central: open_green verified: verified_manual elements_id: 1776571 doi: 10.1016/j.parkreldis.2020.03.014 pii: S1353-8020(20)30070-5 lyricists_name: Kouloulia, Stella lyricists_id: SKOUL07 actors_name: Kalinowski, Damian actors_id: DKALI47 actors_role: owner full_text_status: public publication: Parkinsonism & Related Disorders volume: 73 pagerange: 35-40 event_location: England citation: Emmanouilidou, E; Papagiannakis, N; Kouloulia, S; Galaziou, A; Antonellou, R; Papadimitriou, D; Athanasiadou, A; ... Stefanis, L; + view all <#> Emmanouilidou, E; Papagiannakis, N; Kouloulia, S; Galaziou, A; Antonellou, R; Papadimitriou, D; Athanasiadou, A; Bozi, M; Koros, C; Maniati, M; Vekrellis, K; Ioannou, PC; Stefanis, L; - view fewer <#> (2020) Peripheral alpha-synuclein levels in patients with genetic and non-genetic forms of Parkinson's disease. Parkinsonism & Related Disorders , 73 pp. 35-40. 10.1016/j.parkreldis.2020.03.014 <https://doi.org/10.1016/j.parkreldis.2020.03.014>. Green open access document_url: https://discovery.ucl.ac.uk/id/eprint/10099182/1/Kouloulia_Peripheral%20alpha-synuclein%20levels%20in%20patients%20with%20genetic%20and%20non-genetic%20forms%20of%20Parkinson%27s%20disease_AAM.pdf