TY - JOUR N1 - This article is distributed under the terms of an Attribution?Noncommercial?Share Alike?No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution?Noncommercial?Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/) UR - https://doi.org/10.1084/jem.20190762 A1 - Kang, L A1 - Zhang, X A1 - Ji, L A1 - Kou, T A1 - Smith, SM A1 - Zhao, B A1 - Guo, X A1 - Pineda-Torra, I A1 - Wu, L A1 - Hu, X Y1 - 2020/01/16/ AV - public N2 - Macrophages play pleiotropic roles in maintaining the balance between immune tolerance and inflammatory responses in the gut. Here, we identified transcription factor RBP-J as a crucial regulator of colonic macrophage-mediated immune responses against the enteric pathogen Citrobacter rodentium. In the immune response phase, RBP-J promoted pathogen clearance by enhancing intestinal macrophage-elicited Th17 cell immune responses, which was achieved by maintenance of C/EBP?-dependent IL-6 production by overcoming miRNA-17?92-mediated suppressive effects. RBP-J deficiency-associated phenotypes could be genetically corrected by further deleting miRNA-17?92 in macrophages. In the late phase, noneradicated pathogens in RBP-J KO mice recruited abundant IL-1?-expressing CD64+Ly6C+ colonic macrophages and thereby promoted persistence of ILC3-derived IL-22 to compensate for the impaired innate and adaptive immune responses, leading to ultimate clearance of pathogens. These results demonstrated that colonic macrophage-intrinsic RBP-J dynamically orchestrates intestinal immunity against pathogen infections by interfacing with key immune cells of T and innate lymphoid cell lineages. ID - discovery10097847 VL - 217 IS - 4 JF - The Journal of Experimental Medicine TI - The colonic macrophage transcription factor RBP-J orchestrates intestinal immunity against bacterial pathogens ER -