TY - JOUR PB - WILEY-V C H VERLAG GMBH Y1 - 2017/07/17/ A1 - Tseng, W-H A1 - Chang, C-K A1 - Wu, P-C A1 - Hu, N-J A1 - Lee, G-H A1 - Tzeng, C-C A1 - Neidle, S A1 - Hou, M-H IS - 30 TI - Induced-Fit Recognition of CCG Trinucleotide Repeats by a Nickel-Chromomycin Complex Resulting in Large-Scale DNA Deformation KW - DNA deformation KW - induced-fit recognition KW - neurological disease KW - trinucleotide repeats KW - X-ray crystallography UR - https://doi.org/10.1002/anie.201703989 SP - 8761 AV - public JF - Angewandte Chemie International Edition EP - 8765 SN - 1521-3773 N1 - This version is the author accepted manuscript. For information on re-use, please refer to the publisher's terms and conditions. ID - discovery10096361 VL - 56 N2 - Small?molecule compounds targeting trinucleotide repeats in DNA have considerable potential as therapeutic or diagnostic agents against many neurological diseases. Ni^{II}(Chro)_{2} (Chro=chromomycin A3) binds specifically to the minor groove of (CCG)_{n} repeats in duplex DNA, with unique fluorescence features that may serve as a probe for disease detection. Crystallographic studies revealed that the specificity originates from the large?scale spatial rearrangement of the DNA structure, including extrusion of consecutive bases and backbone distortions, with a sharp bending of the duplex accompanied by conformational changes in the Ni^{II} chelate itself. The DNA deformation of CCG repeats upon binding forms a GGCC tetranucleotide tract, which is recognized by Ni^{II}(Chro)_{2}. The extruded cytosine and last guanine nucleotides form water?mediated hydrogen bonds, which aid in ligand recognition. The recognition can be accounted for by the classic induced?fit paradigm. ER -