eprintid: 10095460 rev_number: 16 eprint_status: archive userid: 608 dir: disk0/10/09/54/60 datestamp: 2020-04-21 11:03:13 lastmod: 2021-11-30 00:35:43 status_changed: 2020-04-21 11:03:13 type: article metadata_visibility: show creators_name: Nichols, JM creators_name: Antolovic, V creators_name: Reich, JD creators_name: Brameyer, S creators_name: Paschke, P creators_name: Chubb, JR title: Cell and molecular transitions during efficient dedifferentiation ispublished: inpress divisions: UCL divisions: B02 divisions: C08 divisions: D77 keywords: developmental biology, dictyostelium, regenerative medicine, stem cells note: © 2020, Nichols et al. This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/). abstract: Dedifferentiation is a critical response to tissue damage, yet is not well understood, even at a basic phenomenological level. Developing Dictyostelium cells undergo highly efficient dedifferentiation, completed by most cells within 24 hours. We use this rapid response to investigate the control features of dedifferentiation, combining single cell imaging with high temporal resolution transcriptomics. Gene expression during dedifferentiation was predominantly a simple reversal of developmental changes, with expression changes not following this pattern primarily associated with ribosome biogenesis. Mutation of genes induced early in dedifferentiation did not strongly perturb the reversal of development. This apparent robustness may arise from adaptability of cells: the relative temporal ordering of cell and molecular events was not absolute, suggesting cell programmes reach the same end using different mechanisms. In addition, although cells start from different fates, they rapidly converged on a single expression trajectory. These regulatory features may contribute to dedifferentiation responses during regeneration. date: 2020-04-07 date_type: published official_url: https://doi.org/10.7554/eLife.55435 oa_status: green full_text_type: other language: eng primo: open primo_central: open_green verified: verified_manual elements_id: 1777305 doi: 10.7554/eLife.55435 pii: 55435 lyricists_name: Antolovic, Vlatka lyricists_name: Chubb, Jonathan lyricists_id: VANTO80 lyricists_id: JRCHU69 actors_name: Chubb, Jonathan actors_id: JRCHU69 actors_role: owner full_text_status: public publication: eLife volume: 9 event_location: England citation: Nichols, JM; Antolovic, V; Reich, JD; Brameyer, S; Paschke, P; Chubb, JR; (2020) Cell and molecular transitions during efficient dedifferentiation. eLife , 9 10.7554/eLife.55435 <https://doi.org/10.7554/eLife.55435>. (In press). Green open access document_url: https://discovery.ucl.ac.uk/id/eprint/10095460/1/elife-55435-v1.pdf