eprintid: 10094889
rev_number: 14
eprint_status: archive
userid: 608
dir: disk0/10/09/48/89
datestamp: 2020-04-16 14:47:37
lastmod: 2021-10-09 23:10:03
status_changed: 2020-04-16 14:47:37
type: article
metadata_visibility: show
creators_name: Bennabi, I
creators_name: Crozet, F
creators_name: Nikalayevich, E
creators_name: Chaigne, A
creators_name: Letort, G
creators_name: Manil-Ségalen, M
creators_name: Campillo, C
creators_name: Cadart, C
creators_name: Othmani, A
creators_name: Attia, R
creators_name: Genovesio, A
creators_name: Verlhac, M-H
creators_name: Terret, M-E
title: Artificially decreasing cortical tension generates aneuploidy in mouse oocytes
ispublished: pub
divisions: UCL
divisions: B02
divisions: C08
divisions: D77
note: This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
abstract: Human and mouse oocytes’ developmental potential can be predicted by their mechanical
properties. Their development into blastocysts requires a specific stiffness window. In this
study, we combine live-cell and computational imaging, laser ablation, and biophysical
measurements to investigate how deregulation of cortex tension in the oocyte contributes to
early developmental failure. We focus on extra-soft cells, the most common defect in a
natural population. Using two independent tools to artificially decrease cortical tension, we
show that chromosome alignment is impaired in extra-soft mouse oocytes, despite normal
spindle morphogenesis and dynamics, inducing aneuploidy. The main cause is a cytoplasmic
increase in myosin-II activity that could sterically hinder chromosome capture. We describe
here an original mode of generation of aneuploidies that could be very common in oocytes
and could contribute to the high aneuploidy rate observed during female meiosis, a leading
cause of infertility and congenital disorders.
date: 2020-04-03
date_type: published
official_url: https://doi.org/10.1038/s41467-020-15470-y
oa_status: green
full_text_type: pub
pmcid: PMC7125192
language: eng
primo: open
primo_central: open_green
verified: verified_manual
elements_id: 1775905
doi: 10.1038/s41467-020-15470-y
pii: 10.1038/s41467-020-15470-y
lyricists_name: Chaigne, Agathe
lyricists_id: ACHAI50
actors_name: Flynn, Bernadette
actors_id: BFFLY94
actors_role: owner
full_text_status: public
publication: Nature Communications
volume: 11
article_number: 1649
event_location: England
citation:        Bennabi, I;    Crozet, F;    Nikalayevich, E;    Chaigne, A;    Letort, G;    Manil-Ségalen, M;    Campillo, C;                         ... Terret, M-E; + view all <#>        Bennabi, I;  Crozet, F;  Nikalayevich, E;  Chaigne, A;  Letort, G;  Manil-Ségalen, M;  Campillo, C;  Cadart, C;  Othmani, A;  Attia, R;  Genovesio, A;  Verlhac, M-H;  Terret, M-E;   - view fewer <#>    (2020)    Artificially decreasing cortical tension generates aneuploidy in mouse oocytes.                   Nature Communications , 11     , Article 1649.  10.1038/s41467-020-15470-y <https://doi.org/10.1038/s41467-020-15470-y>.       Green open access   
 
document_url: https://discovery.ucl.ac.uk/id/eprint/10094889/1/s41467-020-15470-y.pdf