%0 Journal Article
%A Bennabi, I
%A Crozet, F
%A Nikalayevich, E
%A Chaigne, A
%A Letort, G
%A Manil-Ségalen, M
%A Campillo, C
%A Cadart, C
%A Othmani, A
%A Attia, R
%A Genovesio, A
%A Verlhac, M-H
%A Terret, M-E
%D 2020
%F discovery:10094889
%J Nature Communications
%T Artificially decreasing cortical tension generates aneuploidy in mouse oocytes
%U https://discovery.ucl.ac.uk/id/eprint/10094889/
%V 11
%X Human and mouse oocytes’ developmental potential can be predicted by their mechanical  properties. Their development into blastocysts requires a specific stiffness window. In this  study, we combine live-cell and computational imaging, laser ablation, and biophysical  measurements to investigate how deregulation of cortex tension in the oocyte contributes to  early developmental failure. We focus on extra-soft cells, the most common defect in a  natural population. Using two independent tools to artificially decrease cortical tension, we  show that chromosome alignment is impaired in extra-soft mouse oocytes, despite normal  spindle morphogenesis and dynamics, inducing aneuploidy. The main cause is a cytoplasmic  increase in myosin-II activity that could sterically hinder chromosome capture. We describe  here an original mode of generation of aneuploidies that could be very common in oocytes  and could contribute to the high aneuploidy rate observed during female meiosis, a leading  cause of infertility and congenital disorders.
%Z This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.