eprintid: 10094233
rev_number: 24
eprint_status: archive
userid: 608
dir: disk0/10/09/42/33
datestamp: 2020-04-02 13:58:00
lastmod: 2021-10-17 23:43:22
status_changed: 2020-04-02 13:58:00
type: article
metadata_visibility: show
creators_name: Pereira, PM
creators_name: Gustafsson, N
creators_name: Marsh, M
creators_name: Mhlanga, MM
creators_name: Henriques, R
title: Super‐beacons: Open‐source probes with spontaneous tuneable blinking compatible with live‐cell super‐resolution microscopy
ispublished: inpress
divisions: UCL
divisions: B02
divisions: C08
divisions: D77
keywords: DNA, fluorescence microscopy, fluorescence quenching, live-cell, molecular-beacons, super-resolution
note: Copyright © 2020 The Authors. Traffic published by John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
abstract: Localization‐based super‐resolution microscopy relies on the detection of individual molecules cycling between fluorescent and non‐fluorescent states. These transitions are commonly regulated by high‐intensity illumination, imposing constrains to imaging hardware and producing sample photodamage. Here, we propose single‐molecule self‐quenching as a mechanism to generate spontaneous photoswitching. To demonstrate this principle, we developed a new class of DNA‐based open‐source super‐resolution probes named super‐beacons, with photoswitching kinetics that can be tuned structurally, thermally and chemically. The potential of these probes for live‐cell compatible super‐resolution microscopy without high‐illumination or toxic imaging buffers is revealed by imaging interferon inducible transmembrane proteins (IFITMs) at sub‐100 nm resolutions.
date: 2020-03-30
date_type: published
official_url: https://doi.org/10.1111/tra.12728
oa_status: green
full_text_type: pub
language: eng
primo: open
primo_central: open_green
verified: verified_manual
elements_id: 1772674
doi: 10.1111/tra.12728
lyricists_name: Henriques, Ricardo Jose
lyricists_name: Marsh, Mark
lyricists_id: RJSDH70
lyricists_id: MCPMA80
actors_name: Allington-Smith, Dominic
actors_id: DAALL44
actors_role: owner
full_text_status: public
publication: Traffic
event_location: England
citation:        Pereira, PM;    Gustafsson, N;    Marsh, M;    Mhlanga, MM;    Henriques, R;      (2020)    Super‐beacons: Open‐source probes with spontaneous tuneable blinking compatible with live‐cell super‐resolution microscopy.                   Traffic        10.1111/tra.12728 <https://doi.org/10.1111/tra.12728>.    (In press).    Green open access   
 
document_url: https://discovery.ucl.ac.uk/id/eprint/10094233/7/Marsh_tra.12728_VoR.pdf