eprintid: 10092638 rev_number: 20 eprint_status: archive userid: 608 dir: disk0/10/09/26/38 datestamp: 2020-03-04 10:07:45 lastmod: 2021-12-05 00:41:18 status_changed: 2020-03-04 10:07:45 type: article metadata_visibility: show creators_name: Essmann, CL creators_name: Martinez-Martinez, D creators_name: Pryor, R creators_name: Leung, K-Y creators_name: Krishnan, KB creators_name: Lui, PP creators_name: Greene, NDE creators_name: Brown, AEX creators_name: Pawar, VM creators_name: Srinivasan, MA creators_name: Cabreiro, F title: Mechanical properties measured by atomic force microscopy define health biomarkers in ageing C. elegans ispublished: pub divisions: UCL divisions: B02 divisions: D13 divisions: G22 divisions: B04 divisions: C05 divisions: F48 note: Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. abstract: Genetic and environmental factors are key drivers regulating organismal lifespan but how these impact healthspan is less well understood. Techniques capturing biomechanical properties of tissues on a nano-scale level are providing new insights into disease mechanisms. Here, we apply Atomic Force Microscopy (AFM) to quantitatively measure the change in biomechanical properties associated with ageing Caenorhabditis elegans in addition to capturing high-resolution topographical images of cuticle senescence. We show that distinct dietary restriction regimes and genetic pathways that increase lifespan lead to radically different healthspan outcomes. Hence, our data support the view that prolonged lifespan does not always coincide with extended healthspan. Importantly, we identify the insulin signalling pathway in C. elegans and interventions altering bacterial physiology as increasing both lifespan and healthspan. Overall, AFM provides a highly sensitive technique to measure organismal biomechanical fitness and delivers an approach to screen for health-improving conditions, an essential step towards healthy ageing. date: 2020-02-25 date_type: published official_url: http://dx.doi.org/10.1038/s41467-020-14785-0 oa_status: green full_text_type: pub language: eng primo: open primo_central: open_green verified: verified_manual elements_id: 1767551 doi: 10.1038/s41467-020-14785-0 pii: 10.1038/s41467-020-14785-0 lyricists_name: Essmann, Clara lyricists_name: Greene, Nicholas lyricists_name: Leung, Kit-Yi lyricists_name: Pawar, Vijay lyricists_name: Srinivasan, Mandayam lyricists_id: CLESS46 lyricists_id: NDEGR34 lyricists_id: KYLEU59 lyricists_id: VMPAW91 lyricists_id: MASRI64 actors_name: Austen, Jennifer actors_id: JAUST66 actors_role: owner full_text_status: public publication: Nature Communications volume: 11 number: 1 article_number: 1043 event_location: England citation: Essmann, CL; Martinez-Martinez, D; Pryor, R; Leung, K-Y; Krishnan, KB; Lui, PP; Greene, NDE; ... Cabreiro, F; + view all <#> Essmann, CL; Martinez-Martinez, D; Pryor, R; Leung, K-Y; Krishnan, KB; Lui, PP; Greene, NDE; Brown, AEX; Pawar, VM; Srinivasan, MA; Cabreiro, F; - view fewer <#> (2020) Mechanical properties measured by atomic force microscopy define health biomarkers in ageing C. elegans. Nature Communications , 11 (1) , Article 1043. 10.1038/s41467-020-14785-0 <https://doi.org/10.1038/s41467-020-14785-0>. Green open access document_url: https://discovery.ucl.ac.uk/id/eprint/10092638/1/s41467-020-14785-0.pdf