eprintid: 10092638
rev_number: 20
eprint_status: archive
userid: 608
dir: disk0/10/09/26/38
datestamp: 2020-03-04 10:07:45
lastmod: 2021-12-05 00:41:18
status_changed: 2020-03-04 10:07:45
type: article
metadata_visibility: show
creators_name: Essmann, CL
creators_name: Martinez-Martinez, D
creators_name: Pryor, R
creators_name: Leung, K-Y
creators_name: Krishnan, KB
creators_name: Lui, PP
creators_name: Greene, NDE
creators_name: Brown, AEX
creators_name: Pawar, VM
creators_name: Srinivasan, MA
creators_name: Cabreiro, F
title: Mechanical properties measured by atomic force microscopy define health biomarkers in ageing C. elegans
ispublished: pub
divisions: UCL
divisions: B02
divisions: D13
divisions: G22
divisions: B04
divisions: C05
divisions: F48
note: Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
abstract: Genetic and environmental factors are key drivers regulating organismal lifespan but how these impact healthspan is less well understood. Techniques capturing biomechanical properties of tissues on a nano-scale level are providing new insights into disease mechanisms. Here, we apply Atomic Force Microscopy (AFM) to quantitatively measure the change in biomechanical properties associated with ageing Caenorhabditis elegans in addition to capturing high-resolution topographical images of cuticle senescence. We show that distinct dietary restriction regimes and genetic pathways that increase lifespan lead to radically different healthspan outcomes. Hence, our data support the view that prolonged lifespan does not always coincide with extended healthspan. Importantly, we identify the insulin signalling pathway in C. elegans and interventions altering bacterial physiology as increasing both lifespan and healthspan. Overall, AFM provides a highly sensitive technique to measure organismal biomechanical fitness and delivers an approach to screen for health-improving conditions, an essential step towards healthy ageing.
date: 2020-02-25
date_type: published
official_url: http://dx.doi.org/10.1038/s41467-020-14785-0
oa_status: green
full_text_type: pub
language: eng
primo: open
primo_central: open_green
verified: verified_manual
elements_id: 1767551
doi: 10.1038/s41467-020-14785-0
pii: 10.1038/s41467-020-14785-0
lyricists_name: Essmann, Clara
lyricists_name: Greene, Nicholas
lyricists_name: Leung, Kit-Yi
lyricists_name: Pawar, Vijay
lyricists_name: Srinivasan, Mandayam
lyricists_id: CLESS46
lyricists_id: NDEGR34
lyricists_id: KYLEU59
lyricists_id: VMPAW91
lyricists_id: MASRI64
actors_name: Austen, Jennifer
actors_id: JAUST66
actors_role: owner
full_text_status: public
publication: Nature Communications
volume: 11
number: 1
article_number: 1043
event_location: England
citation:        Essmann, CL;    Martinez-Martinez, D;    Pryor, R;    Leung, K-Y;    Krishnan, KB;    Lui, PP;    Greene, NDE;                 ... Cabreiro, F; + view all <#>        Essmann, CL;  Martinez-Martinez, D;  Pryor, R;  Leung, K-Y;  Krishnan, KB;  Lui, PP;  Greene, NDE;  Brown, AEX;  Pawar, VM;  Srinivasan, MA;  Cabreiro, F;   - view fewer <#>    (2020)    Mechanical properties measured by atomic force microscopy define health biomarkers in ageing C. elegans.                   Nature Communications , 11  (1)    , Article 1043.  10.1038/s41467-020-14785-0 <https://doi.org/10.1038/s41467-020-14785-0>.       Green open access   
 
document_url: https://discovery.ucl.ac.uk/id/eprint/10092638/1/s41467-020-14785-0.pdf