eprintid: 10092446 rev_number: 24 eprint_status: archive userid: 608 dir: disk0/10/09/24/46 datestamp: 2021-10-18 13:53:19 lastmod: 2021-10-18 13:53:19 status_changed: 2021-10-18 13:53:19 type: working_paper metadata_visibility: show creators_name: Wee, RWS creators_name: MacAskill, AF title: Biased connectivity of brain-wide inputs to ventral subiculum output neurons ispublished: pub divisions: UCL divisions: B02 divisions: C08 divisions: D09 divisions: G02 note: The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license (http://creativecommons.org/licenses/by-nc-nd/4.0/). abstract: The ventral subiculum (vS) of the mouse hippocampus coordinates diverse behaviours through heterogeneous populations of projection neurons. These neurons transmit signals to multiple brain regions by integrating thousands of local and long-range synaptic inputs. However, whether each population is selectively innervated by different afferent input remains unknown. To address this question, we employed projection-specific rabies tracing to study the input-output relationship of vS output neurons. Analysis of brain-wide inputs reveals quantitative input differences that can be explained by the spatial location of postsynaptic neurons along the proximal-distal axis of vS and the identity of the downstream target. Further, the input from nucleus reuniens, an area thought to underlie vS and prefrontal cortex (PFC) reciprocal connectivity, is unexpectedly biased away from PFC-projecting vS neurons. Overall, we reveal prominent heterogeneity in brain-wide inputs to the vS parallel output circuitry, providing a basis for the selective control of individual projections during behaviour. date: 2019-08-06 date_type: published publisher: BioRxiv official_url: https://doi.org/10.1101/726406 oa_status: green full_text_type: other language: eng primo: open primo_central: open_green verified: verified_manual elements_id: 1684440 doi: 10.1101/726406 lyricists_name: Macaskill, Andrew lyricists_id: AFMAC80 actors_name: Allington-Smith, Dominic actors_id: DAALL44 actors_role: owner full_text_status: public place_of_pub: Cold Spring Harbor, NY, USA pages: 32 citation: Wee, RWS; MacAskill, AF; (2019) Biased connectivity of brain-wide inputs to ventral subiculum output neurons. BioRxiv: Cold Spring Harbor, NY, USA. Green open access document_url: https://discovery.ucl.ac.uk/id/eprint/10092446/7/Macaskill_726406v1.full.pdf