eprintid: 10091585
rev_number: 28
eprint_status: archive
userid: 608
dir: disk0/10/09/15/85
datestamp: 2020-02-28 12:12:16
lastmod: 2021-09-20 22:26:55
status_changed: 2020-02-28 12:12:16
type: article
metadata_visibility: show
creators_name: Angkawinitwong, U
creators_name: Courtenay, AJ
creators_name: Rodgers, A
creators_name: Larraneta, E
creators_name: McCarthy, HO
creators_name: Brocchini, S
creators_name: Donnelly, RF
creators_name: Williams, GR
title: A novel transdermal protein delivery strategy via electrohydrodynamic coating of PLGA microparticles onto microneedles
ispublished: pub
divisions: UCL
divisions: B02
divisions: C08
divisions: D10
divisions: G08
note: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
abstract: Transdermal delivery of biological therapeutics is emerging as a potent alternative to intravenous or subcutaneous injections. The latter come with major challenges including patient discomfort, the necessity for trained personnel, specialized sharps disposal, and risk of infection. Microneedle (MN) technology circumvents many of the abovementioned challenges, delivering biological material directly into the skin and allowing sustained release of the active ingredient both in animal models and in humans. This study describes the use of electrohydrodynamic atomization (EHDA) to coat ovalbumin (OVA)-encapsulated PLGA nanoparticles onto hydrogel-forming MN arrays. The particles showed extended release of OVA over ca. 28 days. Microscopic analysis demonstrated that EHDA could generate a uniform particle coating on the MNs, with 30% coating efficiency. Furthermore, the coated MN array manifested similar mechanical characteristics and insertion properties to the uncoated system, suggesting the coating should have no detrimental effects on the application of the MNs. The coated MNs resulted in no significance increase in anti-OVA specific IgG titres in C57BL/6 mice in vivo as compared to the untreated mice (paired t-test, p >0.05) indicating that the formulations are non-immunogenic. The approach of using EHDA to coat a MN array thus appears to have potential as a novel non-invasive protein delivery strategy.
date: 2020-02-18
date_type: published
publisher: American Chemical Society (ACS)
official_url: https://doi.org/10.1021/acsami.9b22425
oa_status: green
full_text_type: other
language: eng
primo: open
primo_central: open_green
verified: verified_manual
elements_id: 1758206
doi: 10.1021/acsami.9b22425
lyricists_name: Brocchini, Steve
lyricists_name: Williams, Gareth
lyricists_id: SBROC55
lyricists_id: GWILL02
actors_name: Stacey, Thomas
actors_id: TSSTA20
actors_role: owner
full_text_status: public
publication: ACS Applied Materials & Interfaces
volume: 12
number: 11
pagerange: 12478-12488
citation:        Angkawinitwong, U;    Courtenay, AJ;    Rodgers, A;    Larraneta, E;    McCarthy, HO;    Brocchini, S;    Donnelly, RF;           Angkawinitwong, U;  Courtenay, AJ;  Rodgers, A;  Larraneta, E;  McCarthy, HO;  Brocchini, S;  Donnelly, RF;  Williams, GR;   - view fewer <#>    (2020)    A novel transdermal protein delivery strategy via electrohydrodynamic coating of PLGA microparticles onto microneedles.                   ACS Applied Materials & Interfaces , 12  (11)   pp. 12478-12488.    10.1021/acsami.9b22425 <https://doi.org/10.1021/acsami.9b22425>.       Green open access   
 
document_url: https://discovery.ucl.ac.uk/id/eprint/10091585/3/Williams_2020214%20OVA%20coated%20MNs%20paper%20ACS%20AMI%20R2%20no%20tracks.pdf