eprintid: 10090628 rev_number: 31 eprint_status: archive userid: 608 dir: disk0/10/09/06/28 datestamp: 2020-01-31 14:45:50 lastmod: 2021-12-27 23:07:43 status_changed: 2020-01-31 14:45:50 type: article metadata_visibility: show creators_name: Picken, C creators_name: Laing, P creators_name: Shen, L creators_name: Clapp, LH creators_name: Brocchini, S title: Synthetic routes to treprostinil N-acyl methylsulfonamide ispublished: pub divisions: UCL divisions: B02 divisions: C08 divisions: D10 divisions: G08 divisions: D14 divisions: GA2 keywords: Prostacyclin, Treprostinil, Acylsulfonamide, Prodrug note: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions. abstract: The synthesis of the prodrug candidate, treprostinil N-acyl methylsulfonamide 5 was accomplished from treprostinil 2 utilising protecting group strategies. A more direct synthesis for the prodrug was also achieved using a treprostinil triol precursor 12 and bromoacetyl acylmethylsulfonamide 14. The overall yield of treprostinil N-acyl sulfonamide 5 directly from the triol precursor 12 is similar to the protecting group strategies because deprotonation of the acidic proton in the bromoacetyl acylmethylsulfonamide 14 reduces electrophilicity. However, the more direct route using the treprostinil triol precursor holds greater promise as a strategy to prepare a wide range of treprostinil prodrug candidates. Treprostinil N-acyl methylsulfonamide prodrug 5 exhibited a 30-fold decrease in the potency at the human prostacyclin (IP) receptor compared to treprostinil 2 in an in vitro cyclic AMP assay. date: 2020-01-30 date_type: published official_url: https://doi.org/10.1016/j.tetlet.2019.151428 oa_status: green full_text_type: other language: eng primo: open primo_central: open_green verified: verified_manual elements_id: 1733273 doi: 10.1016/j.tetlet.2019.151428 lyricists_name: Brocchini, Steve lyricists_name: Clapp, Lucie lyricists_id: SBROC55 lyricists_id: LCLAP60 actors_name: Dewerpe, Marie actors_id: MDDEW97 actors_role: owner full_text_status: public publication: Tetrahedron Letters volume: 61 number: 5 article_number: 151428 citation: Picken, C; Laing, P; Shen, L; Clapp, LH; Brocchini, S; (2020) Synthetic routes to treprostinil N-acyl methylsulfonamide. Tetrahedron Letters , 61 (5) , Article 151428. 10.1016/j.tetlet.2019.151428 <https://doi.org/10.1016/j.tetlet.2019.151428>. Green open access document_url: https://discovery.ucl.ac.uk/id/eprint/10090628/9/Clapp_Picken%20et%20al_manuscript_clean_cp.pdf