eprintid: 10090448
rev_number: 18
eprint_status: archive
userid: 608
dir: disk0/10/09/04/48
datestamp: 2021-08-09 16:49:03
lastmod: 2021-10-21 23:21:54
status_changed: 2021-08-09 16:49:03
type: working_paper
metadata_visibility: show
creators_name: Pereira, P
creators_name: Gustafsson, N
creators_name: Marsh, M
creators_name: Mhlanga, M
creators_name: Henriques, R
title: Super-Beacons: open-source probes with spontaneous tuneable blinking compatible with live-cell super-resolution microscopy
ispublished: pub
divisions: UCL
divisions: B02
divisions: C08
divisions: D77
note: The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license (http://creativecommons.org/licenses/by/4.0/).
abstract: Localization based super-resolution microscopy relies on the detection of individual molecules cycling between fluorescent and non-fluorescent states. These transitions are commonly regulated by high-intensity illumination, imposing constrains to imaging hardware and producing sample photodamage. Here, we propose single-molecule self-quenching as a mechanism to generate spontaneous photoswitching independent of illumination. To demonstrate this principle, we developed a new class of DNA-based open-source Super-Resolution probes named Super-Beacons, with photoswitching kinetics that can be tuned structurally, thermally and chemically. The potential of these probes for live-cell friendly Super-Resolution Microscopy without high-illumination or toxic imaging buffers is revealed by imaging Interferon Inducible Transmembrane proteins (IFITMs) at sub-100nm resolutions.
date: 2020-02-04
publisher: BioRxiv
official_url: https://doi.org/10.1101/2020.01.20.912311
oa_status: green
full_text_type: pub
language: eng
primo: open
primo_central: open_green
verified: verified_manual
elements_id: 1744615
doi: 10.1101/2020.01.20.912311
lyricists_name: Henriques, Ricardo Jose
lyricists_name: Marsh, Mark
lyricists_id: RJSDH70
lyricists_id: MCPMA80
actors_name: Henriques, Ricardo Jose
actors_id: RJSDH70
actors_role: owner
full_text_status: public
place_of_pub: Cold Spring Harbor, NY, USA
pages: 11
citation:        Pereira, P;    Gustafsson, N;    Marsh, M;    Mhlanga, M;    Henriques, R;      (2020)    Super-Beacons: open-source probes with spontaneous tuneable blinking compatible with live-cell super-resolution microscopy.                    BioRxiv: Cold Spring Harbor, NY, USA.       Green open access   
 
document_url: https://discovery.ucl.ac.uk/id/eprint/10090448/1/2020.01.20.912311v1.full.pdf